环状RNA FLI1在肝细胞癌患者中的表达及其与预后的关系

唐凌; 邱露蝶; 秦文; 吴刚

doi:10.3969/j.issn.1001-5256.2019.09.019

环状RNA FLI1在肝细胞癌患者中的表达及其与预后的关系

DOI: 10.3969/j.issn.1001-5256.2019.09.019

1. 西南医科大学附属医院传染消化科
2. 西南医科大学附属医院中医科

基金项目:

四川省卫生厅基金资助(120326)；

详细信息

中图分类号: R735.7
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出版历程
- 收稿日期: 2019-05-13
- 出版日期: 2019-09-20

Expression of circular RNA FLI1 in patients with hepatocellular carcinoma and its association with prognosis

Department of Infectious Digestion, The Affiliated Hospital of Southwest Medical University

Research funding:

摘要

摘要: 目的探讨环状RNA FLI1（circ FLI1）在肝细胞癌（HCC）患者中的表达及意义。方法收集2013年1月-2018年1月于西南医科大学附属医院经手术切除的45例HCC患者的癌组织及配对癌旁组织和血清样本（HCC组）。同时收集45例健康体检者的血清标本（对照组）。人肝癌细胞株HepG2、SMMC7721、HB611均来自中国科学院生物化学与细胞生物学研究所。real-time PCR法检测circ FLI1在肝癌细胞及45例肝癌组织、血清标本中的表达。计量资料2组间比较采用t检验。采用Kaplan-Meier法分析circ FLI1表达与患者生存时间的关系,采用受试者工作特征曲线（ROC曲线）分析血清circ FLI1 E2-4及circ FLI1 E2-5作为诊断HCC生物标志物的潜能,应用单因素及多因素Cox比例风险模型分析影响HCC预后的因素。结果 circ FLI1 E2-5及circ FLI1 E2-4在3种肝癌细胞中高表达。circ FLI1 E2-4（7. 09±0. 26 vs 1. 14±0. 20,t=19. 970,P <0. 001）及circ FL...
- 癌,肝细胞 /
- 环状RNA FLI1 /
- 基因表达 /
- 预后
Abstract: Objective To investigate the expression and significance of circular RNA FLI1 ( circ FLI1) in patients with hepatocellular carcinoma ( HCC) . Methods A total of 45 HCC patients who underwent surgical resection in The Affiliated Hospital of Southwest Medical University from January 2013 to January 2018 were enrolled as HCC group, and the samples of cancerous tissue, adjacent tissue, and serum were collected. A total of 45 healthy individuals who underwent physical examination were enrolled as control group, and their serum samples were collected. Human hepatoma cell lines HepG2, SMMC7721, and HB611 were obtained from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. qRT-PCR was used to measure the expression of circ FLI1 in hepatoma cells and 45 samples of cancerous tissue and serum. The t-test was used for comparison of continuous data between groups. The Kaplan-Meier method was used to analyze the association between circ FLI1 expression and survival time; the receiver operating characteristic ( ROC) curve was used to evaluate the potential of serum circ FLI1 E2-4 and circ FLI1 E2-5 as the biomarkers for the diagnosis of HCC; univariate and multivariate Cox proportional hazards model analyses were used to investigate the influencing factors for the prognosis of HCC. Results There was high expression of circ FLI1 E2-5 and circ FLI1 E2-4 in the three hepatoma cell lines. Compared with the control group, the HCC group had sig-nificantly higher serum levels of circ FLI1 E2-4 ( 7. 09 ± 0. 26 vs 1. 14 ± 0. 20, t = 19. 970, P < 0. 001) and circ FLI1 E2-5 ( 7. 50 ±0. 25 vs 1. 29 ± 0. 30, t = 16. 640, P < 0. 001) . For the HCC patients, the expression of circ FLI1 E2-4 and circ FLI1 E2-5 in cancerous tissue was significantly higher than that in adjacent tissue ( circ FLI1 E2-4: 7. 62 ± 1. 33 vs 1. 55 ± 0. 32, t = 15. 560, P < 0. 001; circ FLI1 E2-5: 7. 92 ± 0. 35 vs 1. 42 ± 0. 39, t = 21. 170, P < 0. 001) . The patients with high expression of circ FLI1 E2-5 had significantly shorter median progression-free survival time and overall survival time than those with low expression ( median progression-free survival time: 11. 17 ± 0. 49 months vs 23. 35 ± 1. 27 months, χ2= 28. 480, P < 0. 001; overall survival time: 19. 75 ± 0. 76 months vs 37. 44 ± 1. 57 months, χ2= 21. 750, P < 0. 001) . The patients with high expression of circ FLI1 E2-4 had significantly shorter median progression-free survival time and overall survival time than those with low expression ( median progression-free survival time: 10. 29 ± 0. 42 months vs 24.65 ± 1. 58 months, χ2= 19. 620, P < 0. 001; overall survival time: 21. 32 ± 0. 55 months vs 35. 69 ± 1. 74 months, χ2= 19. 730, P < 0.001) . As a serum marker for the diagnosis of HCC, serum circ FLI1 E2-4 had an area under the ROC curve of 0. 910 ( 95% confidence interval [CI]: 0. 621-0. 970, P < 0. 001) , with a sensitivity of 84. 3% and a specificity of 90. 5%. As a serum marker for the diagnosis of HCC, serum circ FLI1 E2-5 had an area under the ROC curve of 0. 760 ( 95% CI: 0. 650-0. 860, P < 0. 001) , with a sensitivity of80. 5% and a specificity of 87. 3%. The multivariate Cox proportional hazards model analysis showed that serum circ FLI1 E2-4 ( hazard ratio [HR]= 3. 060, 95% CI: 1. 630-5. 870, P < 0. 05) and circ FLI1 E2-5 ( HR = 2. 560, 95% CI: 1. 250-6. 460, P < 0. 05) were independent influencing factors for the prognosis of HCC patients. Conclusion Circ FLI1 is highly expressed in the tissue and serum of HCC patients, which is associated with the prognosis of patients. It may be a potential biomarker for prognosis and a therapeutic target for HCC.
- carcinoma, hepatocellular /
- circRNA FLI1 /
- gene expression /
- prognosis

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