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D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用

路欣 刘英 贾蕾 孔令玉 刘殿卿

引用本文:
Citation:

D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用

DOI: 10.3969/j.issn.1001-5256.2019.07.022
基金项目: 

河北省2018年医学科学研究重点课题计划(20181317); 

详细信息
  • 中图分类号: R735.7

Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice

Research funding: 

 

  • 摘要: 目的探讨肝细胞癌大鼠模型肝癌组织中D-双功能蛋白(DBP)的表达及DBP诱导裸鼠肿瘤生长情况。方法 22只雄性SD大鼠随机分为2组,正常对照组大鼠8只,腹腔注射2-乙基亚硝胺诱导肝细胞癌模型组大鼠14只,蛋白免疫印迹、免疫组化和逆转录聚合酶链反应检测DBP表达。14只雄性SPF级BALB/c-nu小鼠,随机分为2组,用空质粒和DBP过表达质粒分别转染HepG2细胞,将2组细胞分别注射于裸鼠皮下,空质粒对照组8只,DBP高表达组6只,检测2组裸鼠成瘤的大小。计量资料2组间比较采用t检验。结果肝细胞癌模型组大鼠肝癌组织中DBP的蛋白表达高于正常大鼠肝脏,差异具有统计学意义(1. 10±0. 35vs 0. 67±0. 12,t=-7. 48,P <0. 05); mRNA表达也高于正常大鼠肝脏,差异具有统计学意义(3. 70±0. 85 vs 1. 17±0. 72,t=-20. 46,P <0. 05)。DBP过表达组的裸鼠肿瘤体积显著大于空质粒组,差异具有统计学差异[(7590. 50±1867. 97) mm3vs (1663. 78±420. 24...

     

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  • 收稿日期:  2019-02-20
  • 出版日期:  2019-07-20
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