HBV cccDNA定量检测方法的研究进展

江培学; 毛日成; 张继明

doi:10.3969/j.issn.1001-5256.2019.06.006

HBV cccDNA定量检测方法的研究进展

DOI: 10.3969/j.issn.1001-5256.2019.06.006

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中图分类号: R512.62
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出版历程
- 收稿日期: 2019-05-09
- 出版日期: 2019-06-20

Recent advances of quantitative detection methods for HBV cccDNA

摘要

摘要: 从慢性HBV感染的肝细胞中清除HBV cccDNA被认为是根除HBV的关键。在抗病毒治疗之前、期间和之后监测HBV cccDNA对于慢性乙型肝炎患者的疗效评估极为重要;随着靶向HBV cccDNA的抗HBV新药研发的不断推进,也迫切需要准确而灵敏的HBV cccDNA检测方法,以评价其疗效。近年来,为了提高HBV cccDNA定量检测的特异度,在传统的PCR定量检测方法的基础上进行了改进。同时,为了提高敏感度,还推出了HBV cccDNA的数字PCR定量检测方法等。本文综述了引入酶切的qPCR法、磁珠捕获杂交法、滚环扩增结合原位PCR法、数字PCR法和单细胞内数字PCR法等方法在HBV cccDNA定量检测方面的应用进展。
- 乙型肝炎病毒 /
- 共价闭合环状DNA /
- 检测方法
Abstract: Elimination of HBV cccDNA from hepatocytes infected with chronic HBV virus is considered to be the key to eradicating HBV.Monitoring HBV cccDNA before, during, and after viral treatment is essential for routine treatment of patients with chronic hepatitis B. With the introduction of new anti-HBV treatment technologies and new drugs targeting HBV cccDNA, Accurate and sensitive HBV cccDNA assays are urgently needed to evaluate efficacy. In recent years, HBV cccDNA detection methods have achieved gratifying results in both traditional PCR methods and digital PCR methods popular in recent years. In this paper, the advances in HBV cccDNA quantitative detection by qPCR, Magnetic bead capture hybridization, rolling circle amplification combined with in situ PCR, digital PCR and digital PCR assay in single cells were reviewed.
- hepatitis B virus /
- cccDNA /
- quantitative detection method

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参考文献(36)

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