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环境内分泌干扰物双酚A对非酒精性脂肪性肝病大鼠模型肠道黏膜屏障的影响

丁雯瑾 袁涛 沈峰 周达 孙超 范建高 陈源文

引用本文:
Citation:

环境内分泌干扰物双酚A对非酒精性脂肪性肝病大鼠模型肠道黏膜屏障的影响

DOI: 10.3969/j.issn.1001-5256.2018.06.027
基金项目: 

国家自然科学基金资助项目(81400610); 教育部留学回国人员科研启动基金项目(201404802); 上海市卫生和计划生育委员会青年科研项目(20134Y043); 上海交通大学医工交叉研究基金资助项目(YG2016MS72); 黎介寿院士肠道屏障研究专项基金(LJS_201214,LJS_201319); 

详细信息
  • 中图分类号: R-332;R575.5

Influence of bisphenol A on intestinal mucosal barrier in rats with nonalcoholic fatty liver disease

Research funding: 

 

  • 摘要:

    目的研究环境内分泌干扰物双酚A(BPA)对非酒精性脂肪性肝病(NAFLD)大鼠炎症反应的影响,以及对肠道黏膜屏障的损害作用。方法将18只成熟雄性SD大鼠随机分为3组:正常组、NAFLD组和NAFLD+BPA组,每组6只。光镜下观察肝脏病理改变,ELISA检测血清TNFα、IL-1β、IL-6和IL-8等炎症因子,鲎试剂终点比色法检测内毒素水平,免疫荧光法观察肠道黏膜Occludin蛋白的表达情况,及实时聚合酶链式反应测定肠道黏膜外周蛋白ZO-1 mRNA的改变。计量资料多组间比较采用单因素方差分析,进一步两两比较用SNK-q检验。结果肝脏病理学证实NAFLD模型建模成功。100 nmol/L BPA摄入8周后,TNFα、IL-6、IL-8炎症因子表达上调[分别为(127.65±22.40)pg/ml、(199.34±17.46)pg/ml和(258.79±12.82)pg/ml]伴内毒素水平增高[(0.88±0.26)EU/ml],与正常组[分别为(64.87±10.83)pg/ml、(91.27±9.82)pg/ml、(123.76±19.68)pg/ml和(0.27±0.09)EU...

     

  • [1]LANG, IA, GALLOWAY TS, SCARLETT A, et al.Association of urinary bisphenol A concentration with medical disorders and laboratory abnormalities in adults[J].JAMA, 2008, 300 (11) :1303-1310.
    [2]MARMUGI A, LASSERRE F, BEUZELIN D, et al.Adverse effects of long-term exposure to bisphenol A during adulthood leading to hyperglycaemia and hypercholesternlemia in mice[J].Toxicology, 2014, 325:133-143.
    [3]LIU Y, MEI C, LIU H, et al.Modulation of cytokine expression in human macrophages by ebdocrine-disrupting chemical BisphenolA[J].Biochem Biophys Res Commun, 2014, 451 (4) :592-588.
    [4]KO A, HWANG MS, PARK JH, et al.Association between urinary bisphenol A and waist circumference in Korean adults[J].Toxicol Res, 2014, 30 (1) :39-44.
    [5]MA Y, XIA W, WANG DQ, et al.Hepatic DNA methylation modifications in early development of rats resulting from perinatal BPA exposure contribute to insulin resistance in adulthood[J].Diabetologia, 2013, 56 (9) :2059-2067.
    [6]ALONSO MAGDALENA P, VIEIRA E, SORIANO S, et al.Bisphenol A exposure during pregnancy disrupts glucose homeostasis in mothers and adult male offspring[J].Environ Health Perspect, 2010, 118 (9) :1243-1250.
    [7]DING WJ, SUN C, SANG YE, et al.Changes of lipid metabolism induced by bisphenol A in rats with NAFLD[J].J Prac Hepatol, 2017, 20 (6) :785-787. (in Chinese) 丁雯瑾, 孙超, 桑玉尔, 等.环境新兴污染物双酚A暴露加重非酒精性脂肪性肝病大鼠脂质代谢异常[J].实用肝脏病杂志, 2017, 20 (6) :785-787.
    [8]LI YP, LUO SM, LENG YZ, et al.Accumulation of adipose tissue macrophages in obese mice induced by bisphenol A[J].Acta Univ Med Anhui, 2016, 51 (10) :1464-1467. (in Chinese) 李应配, 罗时猛, 冷银芝, 等.双酚A诱导肥胖小鼠中脂肪组织巨噬细胞聚集[J].安徽医科大学学报, 2016, 51 (10) :1464-1467.
    [9]XU ZJ, FAN JG, DING XD, et al.Characterization of high-fat, diet-induced, non-alcoholic steatohepatitis with fibrosis in rats[J].Dig Dis Sci, 2010, 55 (4) :931-940.
    [10]HAO XX, ZHANG DZ, YU BY, et al.Reproductive toxicity of bisphenol A in adult male mice and mechanism[J].J Jilin Univ:Med Edit, 2016, 42 (2) :195-199. (in Chinese) 郝兴霞, 张东泽, 于泊洋, 等.双酚A对成年雄性小鼠的生殖毒性及其作用机制[J].吉林大学学报:医学版, 2016, 42 (2) :195-199.
    [11]VOM SAAL FS, HUGHES C.An extensive new literature concerning low-dose effects of bisphenol A shows the need for a new risk assessment[J].Environ Health Perpect, 2005, 113 (8) :926-933.
    [12]SHANKAR A, TEPPALA S.Relationship between urinary bisphenol A levels and diabetes mellims[J].J Clin Endocrinol Metab, 2011, 96 (12) :3822-3826.
    [13]WANG T, LI M, CHEN B, et al.Urinary bisphenol A (BPA) concentration associates with obesity and insulin resistance[J].J Clin Endocrinol Metab, 2012, 97 (2) :e223-e227.
    [14]BATISTA TM, ALONSO MAGDALENA P, VIEIRA E, et al.Shortterm treatment with bisphenol-A leads to metabolic abnormalities in adult male mice[J].PLo S One, 2012, 7 (3) :e33814.
    [15]CASTANEDA-SCEPPA C, CASTANEDA F, CASTANEDA-SCEPPA C, et al.Sodiam-dependent glucose transporter protein as a potential therapeutic target for improving glycemic control in diabetes[J].Nutr Rev, 201l, 69 (12) :720-729.
    [16]LIN Y, DING D, HUANG Q, et al.Downregulation of miR-192causes hepatic steatosis and lipid accumulation by inducing SREBF1:Novel mechanism for bisphenol A-triggered non-alcoholic fatty liver disease[J].Biochim Biophys Acta, 2017, 1862 (9) :869-882.
    [17]LV Q, GAO R, PENG C, et al.Bisphenol A promotes hepatic lipid deposition involving Kupffer cells M1 polarization in male mice[J].J Endocrinol, 2017, 234 (2) :143-154.
    [18]XIA W, JIANG Y, LI Y, et al.Early-life exposure to bisphenol a induces liver injury in rats involvement of mitoehondria-mediated apoptosis[J].PLo S One, 2014, 9 (2) :e90443.
    [19]XIAO L, YANG L.Gut microbiota and nonalcoholic fatty liver disease[J].J Clin Hepatol, 2017, 33 (4) :774-779. (in Chinese) 肖丽, 杨玲.肠道菌群与非酒精性脂肪性肝病的关系[J].临床肝胆病杂志, 2017, 33 (4) :774-779.
    [20]LIU JQ, ZHOU SM.Research advances in intestinal flora in nonalcoholic fatty liver disease[J].J Clin Hepatol, 2017, 33 (12) :2453-2456. (in Chinese) 刘嘉琪, 周少明.非酒精性脂肪性肝病肠道菌群的研究进展[J].临床肝胆病杂志, 2017, 33 (12) :2453-2456.
    [21]DING WJ, SHEN F, FAN JG, et al.Effects of glutamine on the expression of tight junction protein in intestinal epithelium of rats with nonalcoholic fatty liver disease[J].Chin Hepatol, 2014, 19 (9) :673-676. (in Chinese) 丁雯瑾, 沈峰, 范建高, 等.谷氨酰胺对NAFLD大鼠肠道紧密连接蛋白表达与定位的影响[J].肝脏, 2014, 19 (9) :673-676.
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  • 收稿日期:  2017-12-19
  • 出版日期:  2018-06-20
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