HBV相关慢加急性肝衰竭患者血清IL-32和IL-10水平变化及意义

顾静; 王艳; 陈丽; 徐英; 甘建和

doi:10.3969/j.issn.1001-5256.2018.04.021

HBV相关慢加急性肝衰竭患者血清IL-32和IL-10水平变化及意义

DOI: 10.3969/j.issn.1001-5256.2018.04.021

苏州大学附属第一医院感染病科

基金项目:

国家科技部“十二五”重大专项(2012X10002004-008)；

详细信息

中图分类号: R512.62;R575.3
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出版历程
- 出版日期: 2018-04-20

Changes in serum levels of interleukin-32 and interleukin-10 and their clinical significance in patients with HBV-related acute-on-chronic liver failure

Department of Infectious Diseases, The First Affiliated Hospital of Soochow University

Research funding:

摘要

摘要:
目的探讨HBV相关慢加急性肝衰竭（HBV-ACLF）患者血清IL-32和IL-10水平的变化规律及临床意义。方法选取2012年9月-2014年3月在苏州大学附属第一医院住院治疗的38例HBV-ACLF患者、20例慢性乙型肝炎（CHB）患者及20例健康对照者,采用ELISA法动态监测血清IL-32和IL-10水平变化,同时比较HBV-ACLF早中晚期、感染组与非感染组、存活组与死亡组IL-32、IL-10水平。计量资料2组间比较采用t检验,多组间比较采用方差分析,进一步2组间比较采用SNK-q检验。结果 HBV-ACLF组IL-32水平[（500.98±152.33）pg/ml]显著高于CHB组[（281.72±99.28）pg/ml]及健康对照组[（178.16±50.54）pg/ml]（P值均<0.05）;HBV-ACLF组IL-10水平[（4.82±1.03）pg/ml]显著高于CHB组[（3.15±0.98）pg/ml]及健康对照组[（1.62±0.43）pg/ml]（P值均<0.05）。动态监测HBV-ACLF组IL-32水平显示,HBV-ACLF早期高于中期[（5...
- 肝功能衰竭 /
- 肝炎病毒,乙型 /
- 白细胞介素10 /
- 白细胞介素32
Abstract:
Objective To investigate the changes in the serum levels of interleukin-32 (IL-32) and interleukin-10 (IL-10) and their clinical significance in patients with HBV-related acute-on-chronic liver failure (HBV-ACLF) .Methods A total of 38 HBV-ACLF patients who were hospitalized and treated in The First Affiliated Hospital of Soochow University from September 2012 to March 2014 were enrolled as HBV-ACLF group, as well as 20 patients with chronic hepatitis B (CHB) (CHB group) and 20 healthy controls (healthy control group) .ELISA was used for dynamic monitoring of the changes in the serum levels of IL-32 and IL-10.The serum levels of IL-32 and IL-10 were compared between early-, middle-, and late-stage HBV-ACLF groups, between infection group and non-infection group, and between survival group and death group.The t-test was used for comparison of continuous data between two groups; an analysis of variance was used for comparison between multiple groups, and the SNK-q test was used for further comparison between two groups.Results Compared with the CHB group and the healthy control group, the HBV-ACLF group had significantly higher levels of IL-32 (500.98 ± 152.33 pg/ml vs 281.72 ± 99.28 pg/ml and 178.16 ± 50.54 pg/ml, both P < 0.05) and IL-10 (4.82 ± 1.03 pg/ml vs3.15 ± 0.98 pg/ml and 1.62 ± 0.43 pg/ml, both P < 0.05) .Dynamic monitoring of IL-32 level in the HBV-ACLF group showed that the early-stage HBV-ACLF group had a significantly higher level than the middle-stage HBV-ACLF group (540.69 ± 155.71 pg/ml vs 498.43 ± 135.56 pg/ml, P < 0.05) , and the middle-stage HBV-ACLF group had a significantly higher level than the late-stage HBV-ACLF group (498.43 ± 135.56 pg/ml vs 450.77 ± 102.33 pg/ml, P < 0.05) ; as for the level of IL-10, the early-stage HBV-ACLF group had a significantly lower level than the middle-stage HBV-ACLF group (1.94 ± 0.44 pg/ml vs 2.83 ± 0.97 pg/ml, P < 0.05) , and the middle-stage HBV-ACLF group had a significantly lower level than the late-stage HBV-ACLF group (2.83 ± 0.97 pg/ml vs 3.69 ± 1.23 pg/ml, P < 0.05) .The HBV-ACLF infection group had a significantly higher level of IL-32 than the non-infection group (553.41 ± 158.65 pg/ml vs 482.54 ± 110.16 pg/ml, P = 0.021) .Compared with the HBV-ACLF death group, the HBV-ACLF survival group had a significantly lower level of IL-32 (481.95 ± 100.67 pg/ml vs 540.62 ± 112.45 pg/ml, P = 0.011) and a significantly higher level of IL-10 (4.21 ± 1.27 pg/ml vs 3.61 ± 1.05 pg/ml, P = 0.038) .Conclusion The cytokine network plays an important role in the pathogenesis of HBV-ACLF.Serum IL-32 and IL-10 may be involved in disease progression, and dynamic monitoring of their levels helps with prognostic prediction.
- liver failure /
- hepatitis B virus /
- interleukin-10 /
- interleukin-32

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