长链非编码RNA XLOC-007123在乙型肝炎相关肝细胞癌中的表达及临床意义

雷光林; 王权; 程思杰; 赖成材; 王科宇; 严锦; 杨鹏辉; 张绍庚

doi:10.3969/j.issn.1001-5256.2018.02.018

长链非编码RNA XLOC-007123在乙型肝炎相关肝细胞癌中的表达及临床意义

DOI: 10.3969/j.issn.1001-5256.2018.02.018

1. 解放军第三〇二医院肝胆外科中心
2. 军事医学科学院微生物流行病研究所

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中图分类号: R735.7
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- 出版日期: 2018-02-20

Expression of the long non-coding RNA XLOC-007123 in hepatitis B-related hepatocellular carcinoma and its clinical significance

Center of Hepatobiliary Surgery, 302 Hospital of PLA

摘要

摘要:
目的检测长链非编码RNA（lncRNA）XLOC-007123在乙型肝炎相关肝细胞癌（HCC）患者组织（癌、癌旁）及肝癌细胞系中的表达,并分析其表达水平与临床病理特征的关系。方法选取2013年12月-2015年12月于解放军三〇二医院肝胆外科行HCC切除术的52例乙型肝炎相关HCC患者的肝癌组织;同时取同一患者的癌旁组织作为对照。应用荧光实时定量PCR检测lncRNA XLOC-007123在乙型肝炎相关HCC患者癌组织、配对癌旁组织以及肝癌细胞系中的表达水平。其中研究所用细胞系L02、HuH7、HepG2、SMMC-7721均由解放军三○二医院临床管理中心提供。非参数Wilcoxon秩和检验比较lncRNA XLOC-007123在癌组织和配对癌旁组织的表达水平;独立样本t检验比较不同HCC细胞系与正常肝细胞系间lncRNA XLOC-007123表达水平。临床指标差异分析采用χ2检验。结果与癌旁组织相比,lncRNA XLOC-007123在乙型肝炎相关HCC患者的癌组织中表达显著下调（P<0.01）;与正常肝细胞株相比,IncRNA XLOC-0071...
- 癌,肝细胞 /
- 肝炎,乙型 /
- RNA /
- 基因表达
Abstract:
Objective To investigate the expression of the long non-coding RNA (lncRNA) XLOC-007123 in cancer and adjacent tissue, as well as hepatoma cell lines, in patients with hepatitis B-related hepatocellular carcinoma (HCC) , and the association between its expression level and clinicopathological features.Methods A total of 52 patients with hepatitis B-related HCC who underwent resection of HCC in Center of Hepatobiliary Surgery, 302 Hospital of PLA, from December 2013 to December 2015 were enrolled, and HCC tissue samples were collected.Adjacent tissue samples collected from the same patient were used as controls.Quantitative real-time PCR was used to measure the expression of the lncRNA XLOC-007123 in HCC tissue, adjacent tissue, and hepatoma cell lines in the patients with hepatitis B-related HCC.Normal liver cell line LO2 and hepatoma cell lines HuH7, HepG2, and SMMC-7721 were obtained from Research Center for Clinical and Translational Medicine, 302 Hospital of PLA.The nonparametric Wilcoxon rank sum test was used to compare the expression of the lncRNA XLOC-007123 between HCC tissue and adjacent tissue, and the independent samples t-test was used to compare the expression of the lncRNA XLOC-007123 between HCC cell lines and the normal liver cell line.The chi-square test was used for comparison of clinical indices.Results In the patients with hepatitis B-related HCC, the expression of the lncRNA XLOC-007123 was significantly lower in HCC tissue than in adjacent tissue (P < 0.01) ; also, the expression of the lncRNA XLOC-007123 was significantly lower in HuH7, HepG2, and SMMC-7721 cells than in LO2 cells (all P < 0.05) .The expression of the lncRNA XLOC-007123 was associated with the serum level of alpha-fetoprotein (AFP) and tumor stage (χ2= 7.738 and 7.589, both P < 0.05) .Conclusion There is a reduction in the expression of the lncRNA XLOC-007123 in hepatitis B-related HCC tissue, which is associated with the serum level of AFP and pathological staging, suggesting that the lncRNA XLOC-007123 may exert a regulatory effect in the development and progression of hepatitis B-related HCC and may become a potential marker for the diagnosis of hepatitis B-related HCC.
- carcinoma, hepatocellular /
- hepatitis B /
- RNA /
- gene expression

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参考文献(29)

[1]JEMAL A, BRAY F, CENTER MM, et al.Global cancer statistics[J].CA Cancer J Clin, 2011, 61 (2) :69-90.

[2]TORRE LA, BRAY F, SIEGEL RL, et al.Global cancer statistics, 2012[J].CA Cancer J Clin, 2015, 65 (2) :87-108.

[3]SIMARD EP, WARD EM, SIEGEL R, et al.Cancers with increasing incidence trends in the United States:1999 through 2008[J].CA Cancer J Clin, 2012, 62 (2) :118-128.

[4]KOMATSU H.Hepatitis B virus:where do we stand and what is the next step for eradication?[J].World J Gastroenterol, 2014, 20 (27) :8998-9016.

[5]SAHA SK, PARACHONIAK CA, GHANTA KS, et al.Mutant IDH inhibits HNF-4αto block hepatocyte differentiation and promote biliary cancer[J].Nature, 2014, 513 (7516) :110-114.

[6]PROTZER U.Hepatitis:epigenetic control of HBV by HBx protein-releasing the break?[J].Nat Rev Gastroenterol Hepatol, 2015, 12 (10) :558-559.

[7]NECSULEA A, SOUMILLON M, WARNEFORS M, et al.The evolution of lncRNA repertoires and expression patterns in tetrapods[J].Nature, 2014, 505 (7485) :635-640.

[8]ZAVATTARI P, PERRA A, MENEGON S, et al.Nrf2, but notβ-catenin, mutation represents an early event in rat hepatocarcinogenesis[J].Hepatology, 2015, 62 (3) :851-862.

[9]ZHU M, GUO J, LI W, et al.Hepatitis B virus X protein induces expression of alpha-fetoprotein and activates PI3K/m TOR signaling pathway in liver cells[J].Oncotarget, 2015, 6 (14) :12196-12208.

[10]FITZGERALD KA, CAFFREY DR.Long noncoding RNAs in innate and adaptive immunity[J].Curr Opin Immunol, 2014, 26:140-146.

[11]DU Z, SUN T, HACISULEYMAN E, et al.Integrative analyses reveal a long noncoding RNA-mediated sponge regulatory network in prostate cancer[J].Nat Commun, 2016, 7:10982.

[12]WAN L, SUN M, LIU GJ, et al.Long noncoding RNA PVT1 promotes non-small cell lung cancer cell proliferation through epigenetically regulating LATS2 expression[J].Mol Cancer Ther, 2016, 15 (5) :1082-1094.

[13]EDGE SB, COMPTON CC.The American Joint Committee on Cancer:the 7th edition of the AJCC cancer staging manual and the future of TNM[J].Ann Surg Oncol, 2010, 17 (6) :1471-1474.

[14]ZHAI W, SUN Y, JIANG M, et al.Differential regulation of LncRNA-SARCC suppresses VHL-mutant RCC cell proliferation yet promotes VHL-normal RCC cell proliferation via modulating androgen receptor/HIF-2α/C-MYC axis under hypoxia[J].Oncogene, 2017, 36 (31) :4525.

[15]YANG F, ZHANG L, HUO XS, et al.Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans[J].Hepatology, 2011, 54 (5) :1679-1689.

[16]YUAN JH, YANG F, WANG F, et al.A long noncoding RNA activated by TGF-βpromotes the invasion-metastasis cascade in hepatocellular carcinoma[J].Cancer Cell, 2014, 25 (5) :666-681.

[17]IGUCHI T, UCHI R, NAMBARA S, et al.A long noncoding RNA, lncRNA-ATB, is involved in the progression and prognosis of colorectal cancer[J].Anticancer Res, 2015, 35 (3) :1385-1388.

[18]QIU JJ, LIN YY, DING JX, et al.Long non-coding RNA ANRIL predicts poor prognosis and promotes invasion/metastasis in serous ovarian cancer[J].Int J Oncol, 2015, 46 (6) :2497-2505.

[19]LECHEL A, GOUGELET A.Early HCC treatment:a future strategy against interferon/miR-484 axis to revert precancerous lesions?[J].Gut, 2016, 65 (7) :1073-1074.

[20]LUO F, SUN B, LI H, et al.A MALAT1/HIF-2αfeedback loop contributes to arsenite carcinogenesis[J].Oncotarget, 2016, 7 (5) :5769-5787.

[21]YUE B, QIU S, ZHAO S, et al.LncRNA-ATB mediated Ecadherin repression promotes the progression of colon cancer and predicts poor prognosis[J].J Gastroenterol Hepatol, 2016, 31 (3) :595-603.

[22]PANZITT K, TSCHERNATSCH MM, GUELLY C, et al.Characterization of HULC, a novel gene with striking up-regulation in hepatocellular carcinoma, as noncoding RNA[J].Gastroenterology, 2007, 132 (1) :330-342.

[23]LI T, XIE J, SHEN C, et al.Upregulation of long noncoding RNA ZEB1-AS1 promotes tumor metastasis and predicts poor prognosis in hepatocellular carcinoma[J].Oncogene, 2016, 35 (12) :1575-1584.

[24]HE X, BAO W, LI X, et al.The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis[J].Int J Mol Med, 2014, 33 (2) :325-332.

[25]CARR BI, GUERRA V, GIANNINI EG, et al.Significance of platelet and AFP levels and liver function parameters for HCC size and survival[J].Int J Biol Markers, 2014, 29 (3) :e215-e223.

[26]DWYER JP, HOSKING P, LUBEL J.Multiple liver lesions in a patient with positive hepatitis C serology and elevated AFP:is it HCC?[J].Gastroenterology, 2014, 147 (3) :e12-e13.

[27]OHRNBERGER S, THAVAMANI A, BRAEUNING A, et al.Dysregulated serum response factor triggers formation of hepatocellular carcinoma[J].Hepatology, 2015, 61 (3) :979-989.

[28]EL-SERAG HB, MARRERO JA, RUDOLPH L, et al.Diagnosis and treatment of hepatocellular carcinoma[J].Gastroenterology, 2008, 134 (6) :1752-1763.

[29]RODRIGUEZ-PERALVAREZ M, LUONG TV, ANDREANA L, et al.A systematic review of microvascular invasion in hepatocellular carcinoma:diagnostic and prognostic variability[J].Ann Surg Oncol, 2013, 20 (1) :325-339.

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