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肠三叶因子对非酒精性脂肪性肝炎大鼠回肠黏液屏障的影响

梁凯 孔维宗 陈娟 王迎春

引用本文:
Citation:

肠三叶因子对非酒精性脂肪性肝炎大鼠回肠黏液屏障的影响

DOI: 10.3969/j.issn.1001-5256.2017.08.029
详细信息
  • 中图分类号: R575

Effect of trefoil factor 3 on intestinal mucous barrier in rats with nonalcoholic steatohepatitis

  • 摘要:

    目的研究非酒精性脂肪性肝炎(NASH)大鼠肠道黏液屏障改变,探讨肠三叶因子(TFF3)对NASH大鼠肠道黏液屏障的影响,及其对NASH的治疗作用。方法将清洁级雄性SD大鼠60只随机分为正常组、模型组和治疗组,每组20只。正常组给予普通饲料,模型组和治疗组给予高脂饲料12周,诱导NASH模型后,治疗组给予rh TFF3 1 ml·kg-1·d-1(浓度0.1 mg/ml)腹腔内注射,正常组与模型组给予1 ml·kg-1·d-1生理盐水,连续3周。第15周末,FITC标记的右旋糖酐灌胃检测大鼠回肠通透性后,处死大鼠,检测血清AST、ALT、TC、TG、内毒素(ET)水平及二胺氧化酶(DAO)活性。HE染色观察肝脏和回肠末端组织病理变化,PAS染色观察回肠末端杯状细胞并计数,免疫组化方法检测回肠末端紧密连接蛋白Occludin和TFF3蛋白表达水平,实时荧光定量PCR检测TFF3 mRNA转录水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果大鼠血清AST、ALT、TG、T...

     

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  • 收稿日期:  2017-02-22
  • 出版日期:  2017-08-20
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