TROP2、p-ERK1/2和Cyclin D1在胆囊癌组织中的表达及临床意义

李新星; 滕世峰; 徐楷; 张言言; 张卫刚; 张宪文; 姚骏; 姚厚山; 黄歆; 胡志前

doi:10.3969/j.issn.1001-5256.2017.05.024

TROP2、p-ERK1/2和Cyclin D1在胆囊癌组织中的表达及临床意义

DOI: 10.3969/j.issn.1001-5256.2017.05.024

第二军医大学附属长征医院普外一科

基金项目:

国家青年科学基金资助(8140101730)；

详细信息

中图分类号: R735.8
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出版历程
- 出版日期: 2017-05-20

Expression of trophoblast cell-surface antigen 2, phosphorylated extracellular signal-regulated kinase 1/2, and cyclin D1 in gallbladder carcinoma tissue and related clinical significance

First Department of General Surgery, Changzheng Hospital, Second Military Medical University

Research funding:

摘要

摘要:
目的探讨胆囊癌组织中人滋养层细胞表面抗原（TROP）2、磷酸化细胞外信号调节激酶（p-ERK）1/2和细胞周期蛋白D1（Cyclin D1）的表达及其与临床病理参数之间的关系,并分析其与胆囊癌患者预后的关系。方法搜集第二军医大学附属长征医院2005年6月-2010年6月确诊并获取病理标本的胆囊癌患者88例,采用免疫组化法检测88例胆囊癌组织及15例癌旁组织中TROP2、p-ERK1/2和Cyclin D1蛋白的表达。计数资料采用χ2检验,Spearman检验法分析TROP2、p-ERK1/2和Cyclin D1间相关性;单因素和多因素Cox回归分析胆囊癌患者预后的影响因素;Kaplan-Meier法绘制患者的生存曲线。结果 TROP2、p-ERK1/2和Cyclin D1蛋白在胆囊癌组织中的阳性表达率分别为74.30%、58.40%和55.30%,明显高于癌旁组织中的表达（5.42%、35.67%和39.87%）（P值均<0.05）。TROP2、p-ERK1/2和Cyclin D1蛋白的表达与胆囊结石、肿瘤直径、分化程度、血管神经侵犯、淋巴结转移、手术方式及...
- 胆囊肿瘤 /
- 人滋养层细胞表面抗原2 /
- 磷酸化细胞外信号调节激酶1/2 /
- 细胞周期蛋白D1 /
- 预后
Abstract:
Objective To investigate the expression of human trophoblast cell-surface antigen 2 (TROP2) , phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2) , and cyclin D1 in gallbladder carcinoma tissue and their correlation with clinicopathological parameters, as well as their association with the prognosis of patients with gallbladder carcinoma.Methods A total of 88 patients who were diagnosed with gallbladder carcinoma in Changzheng Hospital, Second Military Medical University, from June 2005 to June 2010 were enrolled, and their pathological specimens were obtained.Immunohistochemistry was used to measure the expression of TROP2, p-ERK1/2, and cyclin D1 in 88 gallbladder carcinoma tissue samples and 15 adjacent tissue samples.The chi-square test was used for comparison of categorical data between groups, and the Spearman method was used to investigate the correlation between any two parameters of TROP2, p-ERK1/2, and cyclin D1;univariate and multivariate Cox regression analyses were used to analyze the influencing factors for the prognosis of patients with gallbladder carcinoma;the Kaplan-Meier method was used to plot their survival curves.Results The positive rates of TROP2, p-ERK1/2, and cyclin D1 in gallbladder cancer tissue were 74.30%, 58.40%, and 55.30%, respectively, significantly higher than those in adjacent tissue (5.42%, 35.67% and 39.87%, respectively) (P<0.05) .The expression of TROP2, p-ERK1/2, and cyclin D1 was associated with gallstones, tumor diameter, degree of tumor differentiation, vascular and perineural invasion, lymph node metastasis, surgical procedure, and TNM stage (χ2=4.300 ~ 53.315, all P<0.05) .The expression of TROP2 was positively correlated with that of p-ERK1/2 and cyclin D1 (rs=0.402 and 0.742, both P<0.001) , and the expression of p-ERK1/2 was also positively correlated with that of cyclin D1 (rs=0.242, P=0.023) .The multivariate survival analysis showed that positive expression of TROP2 had a significant influence on 3-year survival rate (RR=2.412, 95% CI:1.186 ~ 5.126, P=0.010) .Conclusion High expression of TROP2 may be an important reason for malignant progression of gallbladder carcinoma, and highly expressed TROP2 may mediate the high expression of p-ERK1/2 and cyclin D1, resulting in the malignant progression of gallbladder carcinoma.Positive expression of TROP2 is an independent risk factor for the prognosis of patients with gallbladder carcinoma and may be an effective target for clinical intervention.
- gallbladder neoplasms /
- human trophoblast cell-surface antigen 2 /
- phosphorylated extracellular signal regulated kinase 1/2 /
- cyclin D1 /
- prognosis

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参考文献(18)

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