HBV相关肝细胞癌肿瘤组织中热休克蛋白mRNA表达水平与肿瘤分期的相关性分析

徐庆年; 陆云飞; 汤伯宗; 陈晓蓉; 杨宗国

doi:10.3969/j.issn.1001-5256.2017.05.015

HBV相关肝细胞癌肿瘤组织中热休克蛋白mRNA表达水平与肿瘤分期的相关性分析

DOI: 10.3969/j.issn.1001-5256.2017.05.015

上海市公共卫生临床中心

基金项目:

上海市卫生和计划生育委员会中医药科研专项课题(2016LP028)； 2015年上海市青年医师培养资助计划；

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中图分类号: R735.7;R512.62
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出版历程
- 出版日期: 2017-05-20

Correlation between mRNA expression of heat shock protein in tumor tissue and tumor stage in patients with HBV-related hepatocellular carcinoma

Shanghai Public Health Clinical Center

Research funding:

摘要

摘要:
目的分析肝细胞癌（HCC）肿瘤组织中热休克蛋白（HSP）mRNA表达水平与HCC肿瘤分期的相关性。方法从NCBI网站的基因表达数据库（GEO）下载GSE14520数据集。从GSE14520基因数据库中获取HSP基因25种,通过与数据库下载的临床资料比对,纳入具有HSP基因mRNA表达水平和有对应临床资料的220例HCC患者。提取220例患者癌组织HSP基因的mRNA表达水平及对应的TNM分期及BCLC分期并进行相关性分析。采用Kruskal-Wallis H非参数检验进行多组间比较,采用单因素及多因素logistic回归分析HSP与HCC肿瘤分期的相关性。结果 HSPA12A、HSPA5、HSP90AA1与HSPA13 mRNA随着TNM分期增高,表达逐渐升高（P值均<0.05）;HSP90AA1 mRNA随着BCLC分期增高,表达逐渐升高（P<0.05）。HSPA4L（OR=1.019,P=0.005）、HSPA12A（OR=1.077,P=0.026）与HSP90AA1（OR=1.001,P=0.002）是影响TNM分期的危险因素;HSPA9 mRNA升高与HCC患者T...
- 热休克蛋白质类 /
- 癌,肝细胞 /
- 肿瘤分期 /
- 回归分析
Abstract:
Objective To investigate the correlation between mRNA expression of heat shock protein (HSP) and tumor stage in patients with HBV-related hepatocellular carcinoma (HCC) .Methods The GSE14520 data set was downloaded from gene expression omnibus in the website of the National Center of Biotechnology Information.A total of 25 HSP genes were obtained from the GSE14520 data, and after the comparison with the clinical data downloaded from the database, 220 HCC patients with mRNA expression of HSP and corresponding clinical data were enrolled.The mRNA expression of HSP in tumor tissues was measured, the TNM stage and Barcelona Clinic Liver Cancer (BCLC) stage were determined for each patient, and a correlation analysis was performed.The Kruskal-Wallis H non-parametric test was used for comparison between multiple groups, and univariate and multivariate logistic regression analyses were used to investigate the correlation between HSP and HCC tumor stage.Results With the increase in TNM stage, the expression of HSPA12 A, HSPA5, HSP90AA1, and HSPA13 gradually increased (all P<0.05) , while the expression of HSP90AA1 gradually increased with the increase in BCLC stage (P<0.05) .HSPA4L (OR=1.019, P=0.005) , HSPA12A (OR=1.077, P=0.026) , and HSP90AA1 (OR=1.001, P=0.002) were risk factors for TNM stage.HSPA9 was negatively correlated with TNM stage (OR=0.995, P=0.011) .HSPH1 (OR=1.005, P=0.041) , HSPA1B (OR=1.002, P=0.048) , and HSPA5 (OR=1.000, P=0.001) were risk factors for BCLC stage, and HSPA9 was negatively correlated with BCLC stage (OR=0.995, P=0.005) .Conclusion There is a certain correlation between HSPs and tumor stage in HCC patients.
- heat-shock proteins /
- carcinoma, hepatocellular /
- neoplasm staging /
- regression analysis

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