苦参碱治疗非酒精性脂肪性肝炎大鼠的效果观察

艾正琳; 谢步善; 姚树坤

doi:10.3969/j.issn.1001-5256.2016.11.033

苦参碱治疗非酒精性脂肪性肝炎大鼠的效果观察

DOI: 10.3969/j.issn.1001-5256.2016.11.033

首都医科大学附属北京地坛医院消化内科

详细信息

中图分类号: R259
计量
- 文章访问数: 2134
- HTML全文浏览量: 11
- PDF下载量: 493
- 被引次数: 0
出版历程
- 出版日期: 2016-11-20

Clinical effect of matrine in treatment of rats with nonalcoholic steatohepatitis

摘要

摘要:
目的研究苦参碱的抗氧化作用,并观察其治疗非酒精性脂肪性肝炎（NASH）大鼠的疗效。方法 30只大鼠随机分为3组:对照组、NASH组和苦参碱组,每组10只。采用高脂饮食诱导建立NASH大鼠模型,并给予苦参碱36 mg·kg-1·d-1灌胃治疗。观察所有大鼠体质量、肝重变化,并采用HE染色观察各组大鼠肝组织病理学改变,测定血清ALT、AST水平及肝组织还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）活性。多组间比较采用单因素方差分析,进一步两两比较采用SNK-q检验。结果与对照组比较,NASH组、苦参碱组的大鼠体质量均明显增加（P值均<0.001）,肝重均明显减轻（P值均<0.001）。苦参碱组大鼠肝组织病理学改变较NASH组亦明显减轻。苦参碱组大鼠ALT、AST水平与NASH组比较均有所降低,差异均有统计学意义[（52.0±3.0）U/L vs（41.8±3.7）U/L;（233.6±9.4）U/L vs（170.1±1.8）U/L,P值均<0.001)]。苦参碱组与NASH组比较,大鼠肝组织SOD、GSH水平明显升高[（17.7±2.0）μg/mg...
- 脂肪肝 /
- 苦参碱 /
- 抗氧化剂
Abstract:
Objective To investigate the antioxidant effect of matrine and its clinical effect in the treatment of rats with nonalcoholic steatohepatitis( NASH). Methods A total of 30 rats were randomly divided into three groups: control group,NASH group,and matrine group,with 10 in each. A high- fat diet was used to establish the rat model of NASH,and matrine was given by gavage for treatment at a dose of36 mg·kg- 1·d- 1. The changes in body weight and liver weight were observed in all rats. HE staining was used to observe the histopathological changes of the liver. The serum levels of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) and the content of reduced glutathione( GSH),superoxide dismutase( SOD),and malondialdehyde( MDA) in liver tissue were measured. A one- way analysis of variance was used for the comparison between multiple groups,and the SNK- q test was used for further comparison between any two groups. Results Compared with the NASH group,the matrine group had significant reductions in the serum levels of ALT and AST( ALT:52. 0 ± 3. 0 U / L vs 41. 8 ± 3. 7 U / L,P < 0. 001; AST: 233. 6 ± 9. 4 U / L vs 170. 1 ± 1. 8 U / L,P < 0. 001). The matrine group showed marked improvement in the histopathological changes of the liver compared with the NASH group. Compared with the NASH group,the matrine group had significantly increased content of SOD and GSH in liver tissue( SOD: 17. 7 ± 2. 0 μg / mg vs 27. 0 ± 3. 6 μg / mg,P < 0. 001;GSH: 16. 5 ± 1. 6 U / mg vs 28. 5 ± 2. 1 U / mg,P < 0. 001) and significantly reduced content of MDA( 22. 9 ± 1. 9 nmol / mg vs 17. 8 ± 1. 8nmol / mg,P < 0. 001). Conclusion Matrine has an antioxidant effect and a marked clinical effect in the treatment of rats with NASH.
- fatty liver /
- matrine /
- antioxidants

HTML全文

参考文献(10)

[1]SCHWENGER KJ,ALLARD JP.Clinical approaches to non-alcoholic fatty liver disease[J].World J Gastroenterol,2014,20(7):1712-1723.

[2]ARULANANDAN A,LOOMBA R.Non-invasive testing for NASH and NASH with advanced fibrosis:Are we there yet?[J].Curr Hepatol Rep,2015,14(2):109-118.

[3]MA JM,WANG JQ.Treatment of non-alcoholic fatty liver disease from deficiency[J].J Changchun Univ Tradit Chin Med,2014,30(10):850-852.(in Chinese)马菁蔓,王景泉.从虚论治非酒精性脂肪肝[J].长春中医药大学学报,2014,30(10):850-852.

[4]LE TA,LOOMBA R.Management of non-alcoholic fatty liver disease and steatohepatitis[J].J Clin Exp Hepatol,2012,2(2):156-173.

[5]DAY CP,JAMES OF.Steatohepatitis:a tale of two“hits”?[J].Gastroenterology,1998,114(4):842-845.

[6] The Chinese National Workshop on Fatty Liver and Alcoholic Liver disease for the Chinese Liver Disease Association.Guidelines for management of nonalcoholic fatty liver disease:an updated and revised edition[J].J Clin Hepatol,2010,26(2):120-124.(in Chinese)中华医学会肝病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南(2010年修订版)[J].临床肝胆病杂志,2010,26(2):120-124.

[7]CALZADILLA BL,ADAMS LA.The natural course of nonalcoholic fatty liver disease[J].Int J Mol Sci,2016,17(5):774.

[8]XIONG J,WANG J,HUANG J,et al.Non-alcoholic steatohepatitis-related liver cirrhosis is increasing in China:a tenyear retrospective study[J].Clinics(Sao Paulo),2015,70(8):563-568.

[9]GONG S,SUN JM,TIAN LL,et al.Research progress of the anti-tumor effect of alkaloids of natural medicine[J].Jilin J Tradit Chin Med,2014,34(9):927-931.(in Chinese)宫爽,孙佳明,田淋淋,等.天然药物中生物碱类成分抗肿瘤活性[J].吉林中医药,2014,34(9):927-931.

[10]SHEN H,NIE HM,CHEN YY,et al.Status of treatments of traditional on chronic hepatitis B[J].J Changchun Univ Chin Med,2015,31(4):873-876.(in Chinese)申弘,聂红明,陈逸云,等.慢性乙型肝炎的中医药治疗[J].长春中医药大学学报,2015,31(4):873-876.

施引文献

资源附件(0)

访问统计