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Potential clinical significance of serum HBV RNA virus-like particles
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摘要:
最近的工作证实了慢性乙型肝炎患者血清中存在的HBV RNA为3.5 kb大小的前基因组RNA(pgRNA),这些pgRNA存在于形态结构类似于丹颗粒的病毒样颗粒中。由于pgRNA直接以肝细胞核内的病毒共价闭合环状DNA(ccc DNA)为模板转录,其产生过程不受核苷和核苷酸类药物的抑制。据此推测,血清中病毒RNA的存在应能够反映接受治疗的患者肝细胞内ccc DNA的存在及转录活性,故可作为一个潜在的指导患者核苷和核苷酸类药物安全停药的指标。基于小样本停药队列的结果显示停药点血清中HBV RNA的存在的确与停药后的病毒学反弹相关。
Abstract:Our recent studies confirmed that the HBV RNAs present in the serum of chronic hepatitis B( CHB) patients are pregenomic RNAs( pgRNAs) with a size of 3. 5 kb. These pgRNAs are located in virus- like particles whose morphological structure is similar to that of Dan granules. Since pgRNAs can only be transcribed from the covalently closed circular DNA( ccc DNA) in virus located in the nuclear of infected hepatocytes,and the production of pgRNAs is not affected by nucleos( t) ide analogues( NAs),the presence of viral RNA in serum can reflect the presence of ccc DNA in hepatocytes and its transcriptional activity in patients treated with medication. It can be inferred that serum viral RNA can be used as a potential index for safe withdrawal of NAs. Our results based on a small- sample cohort show that the presence of HBV RNA in serum at the time of withdrawal is indeed associated with virological rebound after drug withdrawal.
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Key words:
- hepatitis B,chronic /
- viral load
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