乙型肝炎肝硬化患者外周血自然杀伤细胞频率、功能及其受体表达的变化

张爱民; 柳芳芳; 杨晓; 李文刚; 韩萍; 段学章

doi:10.3969/j.issn.1001-5256.2016.06.020

乙型肝炎肝硬化患者外周血自然杀伤细胞频率、功能及其受体表达的变化

DOI: 10.3969/j.issn.1001-5256.2016.06.020

解放军第三O二医院肿瘤放射治疗中心

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首都特色课题(Z151100004015002)；

详细信息

中图分类号: R512.62;R575.2
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出版历程
- 出版日期: 2016-06-20

Changes in natural killer cell frequency,function,and receptor expression in peripheral blood of patients with hepatitis B cirrhosis

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摘要

摘要: 目的调查乙型肝炎肝硬化患者外周血自然杀伤细胞（NK细胞）频率、功能及受体表达的变化。方法收集2013年6月-2014年12月解放军第三○二医院收治的乙型肝炎肝硬化患者34例作为肝硬化组,另收集30例健康献血者为健康对照组,用流式细胞仪分析两组外周血NK细胞频率及其受体CD158a、CD158b、NKG2D、NKP30、NKP44、NKP46的表达情况,用白细胞介素（IL）12刺激外周血单个核细胞、流式细胞术检测NK细胞分泌干扰素（IFN）γ和肿瘤坏死因子（TNF）α的能力,并用流式细胞分析法检测NK细胞对K562细胞的杀伤效率,对两组NK细胞频率、受体表达及功能进行分析和比较。正态分布资料两组间比较采用t检验,非正态分布资料两组间比较采用Mann-Whitney U检验。结果 CD56dimNK细胞频率肝硬化组低于健康对照组,差异有统计学意义（Z=-2.309,P<0.05）,CD56brightNK细胞频率肝硬化组高于健康对照组,差异有统计学意义（Z=2.395,P<0.05）。CD56dimNK细胞的活化性受体NKP44、NKP46表达率肝硬化组高于健康对照组,差异均有...
- 肝硬化 /
- 杀伤细胞,天然 /
- 受体,细胞表面
Abstract: Objective To investigate the changes in natural killer(NK) cell frequency,function,and receptor expression in the peripheral blood of patients with hepatitis B cirrhosis.Methods A total of 34 patients with hepatitis B cirrhosis who were admitted to 302 Hospital of PLA from June 2013 to December 2014 were enrolled as the cirrhosis group,and 30 healthy blood donors were enrolled as the healthy control group.Flow cytometry was used to measure NK cell frequency and the expression of CD158 a,CD158b,NKG2 D,NKP30,NKP44,and NKP46 receptors in peripheral blood.Interleukin- 12(IL- 12) was used to stimulate peripheral blood mononucleated cells,and flow cytometry was used to measure the ability of NK cells to secrete interferon- γ(IFNγ) and tumor necrosis factor- α(TNFα) and analyze the killing efficiency of NK cells for K562 cells.NK cell frequency,receptor expression,and function were compared between the two groups.The t- test was used for comparison of normally distributed data between groups,and the Mann- Whitney U rank sum test was used for comparison of abnormally distributed data between groups.Results Compared with the healthy control group,the cirrhosis group had a significantly lower CD56 dimNK cell frequency(Z =- 2.309,P < 0.05) and a significantly higher CD56 brightNK cell frequency(Z = 2.395,P < 0.05).The cirrhosis group had significantly higher expression rates of NKP44 and NKP46(the activating receptors of CD56 dimNK cells) than the healthy control group(Z = 2.834 and 3.404,both P < 0.05).The cirrhosis group also had significantly higher expression rates of NKP30,NKP44,and NKP46(the receptors of CD56 brightNK cells) than the healthy control group(Z = 3.518,4.003,and 4.480,all P <0.05).After IL- 12 stimulation,compared with the healthy control group,the cirrhosis group had significantly lower expression rates of TNFα in CD56 dimNK cells and IFNγ in CD56 brightNK cells(Z =- 3.510 and- 4.301,both P < 0.05).With effector- target ratios of 3∶ 1,10∶ 1,and 30∶ 1,the cirrhosis group had significantly lower proportions of K562 cells killed by NK cells than the healthy control group(Z =- 2.355,- 2.523,and- 2.523,all P < 0.05).Conclusion The patients with liver cirrhosis experience a reduced NK cell function,which may be related to reduced frequency of CD56 dimNK cells and secretion of cytokines,but may not be related to receptor expression.
- liver cirrhosis /
- killer cells /
- natural

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参考文献(9)

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