梗阻性黄疸患者血清补体C5a水平与肝纤维化标志物的相关性

李长政; 刘全达; 于慧杰; 胡文炜; 李雪; 刘明浩

doi:10.3969/j.issn.1001-5256.2016.03.020

梗阻性黄疸患者血清补体C5a水平与肝纤维化标志物的相关性

DOI: 10.3969/j.issn.1001-5256.2016.03.020

1. 火箭军总医院消化科
2. 火箭军总医院肝胆外科
3. 火箭军总医院中心实验室

基金项目:

国家自然科学基金资助项目(30971355)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2016-03-20

Correlation between serum levels of complement C5a and liver fibrosis markers in patients with obstructive jaundice

Department of Gastroenterology, The General Hospital of PLA Rocket Force

Research funding:

摘要

摘要: 目的探讨梗阻性黄疸患者梗阻解除前后血清补体C5a水平的变化情况及其与肝纤维化标志物的相关性。方法收集2012年6月-2014年6月在本院行经内镜逆行胰胆管造影的梗阻性黄疸患者164例,选取20例健康人作为对照组。检测血清补体C5a水平和Ⅳ型胶原、Ⅲ型前胶原、层黏连蛋白、透明质酸4项肝纤维化标志物水平。组间比较采用t检验,相关性分析采用线性相关分析。结果梗阻性黄疸患者血清补体C5a水平较健康对照人群升高[（89.7±30.2）vs（62.2±21.1）ng/L,t=2.213,P=0.016),并与梗阻病程密切相关（r=0.954,P=0.003）。解除梗阻后血清补体C5a水平降至（66.2±26.3）ng/L,与解除前相比差异有统计学意义（t=1.998,P=0.021）。解除梗阻前与血清补体C5a水平呈同步升高相关性的有Ⅳ型胶原（r=0.976,P<0.001）、Ⅲ型前胶原（r=0.972,P<0.001）、层黏连蛋白（r=0.915,P=0.039）和透明质酸（r=0.962,P=0.002）;解除梗阻后与血清补体C5a水平呈同步下降相关性的有Ⅳ型胶原（r=0.965,...
- 肝硬化 /
- 黄疸,阻塞性 /
- 补体C5a
Abstract: Objective To investigate the change in the serum level of complement C5 a after obstruction removal and its correlation with the serum levels of liver fibrosis markers in patients with obstructive jaundice. Methods A total of 164 patients with obstructive jaundice who underwent endoscopic retrograde cholangiopancreatography and obstruction removal in our hospital from June 2012 to June 2014 were enrolled,and 20 healthy adults were enrolled as the control group. The serum levels of complement C5 a and four liver fibrosis markers,type Ⅳcollagen( ⅣP),pre- type Ⅲ collagen( PⅢP),laminin( LN),and hyaluronic acid( HA),were measured. The t- test was applied for comparison between groups,and linear correlation analysis was applied for correlation analysis. Results Compared with the healthy controls,the patients with obstructive jaundice had a significantly higher serum level of complement C5a( 89. 7 ± 30. 2 vs 62. 2 ± 21. 1 ng / L;t = 2. 213,P = 0. 016),and the serum level of complement C5 a was closely related to the course of obstruction( r = 0. 954,P = 0. 003).The serum level of complement C5 a decreased significantly after obstruction removal( 66. 2 ± 26. 3 ng / L; t = 1. 998,P = 0. 021). Before obstruction removal,the serum level of complement C5 a increased synchronously with those of ⅣP( r = 0. 976,P < 0. 001),PⅢP( r =0. 972,P < 0. 001),LN( r = 0. 915,P = 0. 039),and HA( r = 0. 962,P = 0. 002); after obstruction removal,the serum level of complement C5 a decreased synchronously with those of ⅣP( r = 0. 965,P = 0. 001),PⅢP( r = 0. 912,P = 0. 003),and HA( r = 0. 875,P =0. 023). Conclusion Complement C5 a may be involved in the development of liver fibrosis induced by obstructive jaundice.
- liver cirrhosis /
- jaundice,obstructive /
- complement C5a

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参考文献(11)

[1]LI CZ,HU WW,LI W,et al.Effect of time of biliary drainage on biliary obstructive liver fibrosis in rats[J].J Clin Hepatol,2011,27(9):944-946.(in Chinese)李长政,胡文炜,李闻,等.胆汁引流时机对大鼠胆管阻塞性肝纤维化的影响[J].临床肝胆病杂志,2011,27(9):944-946.

[2]SCHMITT J,RODERFELD M,SABRANE K,et al.Complement factor C5 deficiency significantly delays the progression of biliary fibrosis in bile duct-ligated mice[J].Biochem Biophys Res Commun,2012,418(3):445-450.

[3]LI CZ,GUO SL.LI QS,et al.Polymorphism of complement C5gene and susceptibility to liver cirrhosis in patients with chronic hepatitis B[J].J Pract Hepatol,2011,14(6):427-429.(in Chinese)李长政,郭水龙,李青山,等.补体C5a基因多态性与肝硬化发病关系的研究[J].实用肝脏病杂志,2011,14(6):427-429.

[4]RODRIGUEZ-OSORIO L,ORTIZ A.Timing of eculizumab therapy for C3 glomerulonephritis[J].Clin Kidney J,2015,8(4):449-452.

[5]MERLE NS,NOE R,HALBWACHS-MECARELLI L,et al.Complement system partⅡ:role in immunity[J].Front Immunol,2015,6:257.

[6]WONG GL.Prediction of fibrosis progression in chronic viral hepatitis[J].Clin Mol Hepatol,2014,20(3):228-236.

[7]ZHOU Y,XIONG HP,CHEN Q,et al.Relationship between serum markers and severity of liver fibrosis in patients with chronic hepatitis D[J].J Clin Hepatol,2015,31(3):361-364.(in Chinese)周颖,熊红品,陈铿,等.血清肝纤维化标志物与慢性丁型肝炎肝纤维化程度关系的探讨[J].临床肝胆病杂志,2015,31(3):361-364.

[8]DAS D,BARNES MA,NAGY LE.Anaphylatoxin C5a modulates hepatic stellate cell migration[J].Fibrogenesis Tissue Repair,2014,7:9.

[9]GU H,MICKLER EA,CUMMINGS OW,et al.Crosstalk between TGF-β1 and complement activation augments epithelial injury in pulmonary fibrosis[J].FASEB J,2014,28(10):4223-4234.

[10]DANOBEITIA JS,DJAMALI A,FERNANDEZ LA.The role of complement in the pathogenesis of renal ischemia-reperfusion injury and fibrosis[J].Fibrogenesis Tissue Repair,2014,7:16.

[11]XU GL,CHEN J,YANG F,et al.C5a/C5aR pathway is essential for the pathogenesis of murine viral fulminant hepatitis by way of potentiating Fgl2/fibroleukin expression[J].Hepatology,2014,60(1):114-124.

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