PARP-1和caspase-3在胰腺癌及癌旁组织中的表达及意义

蒲竞; 李汛; 朱骏; 白仲天; 严峻

doi:10.3969/j.issn.1001-5256.2016.02.029

PARP-1和caspase-3在胰腺癌及癌旁组织中的表达及意义

DOI: 10.3969/j.issn.1001-5256.2016.02.029

1. 甘肃省兰州市第二人民医院普外三科
2. 兰州大学第一附属医院普外二科

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中图分类号: R735.9
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出版历程
- 收稿日期: 2015-06-04
- 出版日期: 2016-02-20

Expression and roles of poly(ADP-ribose) polymerase-1 and caspase-3 in pancreatic cancer and adjacent tissues

Third Department of General Surgery,The Second People's Hospital of Lanzhou

摘要

摘要: 目的探讨聚（腺苷酸二磷酸核糖）聚合酶（PARP）1和半胱氨酸天冬氨酸蛋白酶（caspase）3在胰腺癌及胰腺癌旁组织中的表达。方法收集2013年1月-2014年6月于兰州大学第一附属医院行手术治疗并经术后病理证实为胰腺癌患者的癌组织标本66例及癌旁组织标本113例,行免疫组化检查,分别检测标本中PARP-1及caspase-3的表达。计数资料组间比较采用χ2检验。结果 66例胰腺癌标本中53例有不同程度PARP-1表达,阳性率为80.3%;113例癌旁组织中有39例PARP-1表达,阳性率为34.5%;PARP-1在胰腺癌组织中的阳性表达率明显高于癌旁组织,差异具有统计学意义（χ2=34.79,P<0.01）。在中分化（18/25,72.0%）和低分化（10/14,71.4%）的胰腺癌组织中PARP-1的表达强度明显高于高分化（3/14,21.4%）肿瘤组织,三者间差异具有统计学意义（χ2=10.76,P<0.01）。66例胰腺癌标本中47例有不同程度caspase-3表达,阳性率为71.2%;113例癌旁组织中有71例caspase-3表达,阳性率为62.8%。高分化（1...
- 胰腺肿瘤 /
- 聚ADP核糖聚合酶类 /
- 半胱氨酸天冬氨酸蛋白酶3
Abstract: Objective To investigate the protein expression of poly( ADP- ribose) polymerase- 1( PARP- 1) and caspase- 3 in pancreatic cancer and adjacent tissues. Methods A total of 66 cancer tissue samples and 113 samples of adjacent tissues were collected from the patients who underwent surgical treatment with a confirmed diagnosis of pancreatic cancer by postoperative pathological examinations in The First Hospital of Lanzhou University from January 2013 to June 2014,and immunohistochemical examinations were performed to measure the expression of PARP- 1 and caspase- 3 in these samples. The chi- square test was applied for comparison of categorical data between groups. Results Of all the 66 samples of pancreatic cancer,53 had varying degrees of PARP- 1 expression,with a positive rate of80. 3%; of all the 133 samples of adjacent tissues,39 had PARP- 1 expression,with a positive rate of 34. 5%; the expression of PARP-1 in the samples of pancreatic cancer was significantly higher than that in the samples of adjacent tissues( χ2= 34. 79,P < 0. 01). The expression of PARP- 1 in moderately and poorly differentiated samples of pancreatic cancer( 18 /25,72%; 10 /14,71. 4%) was significantly higher than that in highly differentiated samples( 3 /14,21. 4%)( χ2= 10. 76,P < 0. 01). Of all the 66 samples of pancreatic cancer,47 had varying degrees of caspase- 3 expression,with a positive rate of 71. 2%; of all the 133 samples of adjacent tissues,71 had caspase-3 expression,with a positive rate of 62. 8%. The expression of caspase- 3 in highly differentiated samples of pancreatic cancer( 11 /18,61. 1%) was significantly higher than that in moderately and poorly differentiated samples( 4 /20,20%; 0 /9,0)( χ2= 11. 44,P < 0. 01).Conclusion The expression level of PARP- 1 in the samples of pancreatic cancer is significantly higher than that in the samples of adjacent tissues,and the strong positive rate of PARP- 1 increases with the decrease in the degree of differentiation; the expression of caspase- 3 is similar between pancreatic cancer and adjacent tissues,and the strong positive rate of caspase- 3 decreases with the decrease in the degree of differentiation. The expression levels of PARP- 1 and caspase- 3 may be related to the development and progression of pancreatic cancer.
- pancreatic neoplasms /
- poly(ADP-ribose) polymerases /
- caspase 3

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参考文献(19)

[1] TAN J.Analysis of the lastest incidence and fatality rate of tumors in China and the rest of the world[R].Chin Cancer Registry Annu Rep,2013.(in Chinese)谭健.中国和全球最新肿瘤发病率和死亡率解析[R].中国肿瘤登记年报,2013.

[2]FENG JJ,JIA GZ,TAN GY,et al.Changes of serum level of carbohydrate antigen CA19-9 in patients with liver diseases and its clinical significance[J].The J Pract Med,2008,25(9):1043-1044.(in Chinese)逢金聚,贾桂芝,谭国英,等.肝病患者肿瘤标记物CA199水平变化及其临床意义[J].实用医学杂志,2008,25(9):1043-1044.

[3]KIEBACK DG.Adenovirus-mediated thymidine kinase gene therapy induces apoptosis in human epithelial O-varian cancer cells and damages PARP-1[J].In Vivo,2009,23(1):77-80.

[4]CHO BB,TOLEDO-PEREYRA LH.Caspase-independent programmed cell death following ischemic stroke[J].J Invest Surg,2008,21(3):141-147.

[5]NOSHO K,YAMAMOTO H,MIKAMI M,et al.Overexpression of poly(ADP-ribose)polymerase-1(PARP-1)in the early stage of colorectal carcinogenesis[J].Eur J Cancer,2006,42(14):2374-2381.

[6]OSSOVSKAYA V,KOO IC,KALDJIAN EP,et al.Upregulation of poly(ADP-ribose)polymerase-1(PARP-1)in triple-negative breastcancer and other primary human tumor types[J].Genes Cancer,2010,1(8):812-821.

[7]LI Y,WEI W,LYU SQ,et al.Effects of PARP-1 on angiogenesis of ovarian cancer[J].J Shandong Univ:Health Sciences,2014,52(4):97-106.(in Chinese)李燕,尉蔚,吕树卿,等.PARP-1对卵巢癌血管生成的影响[J].山东大学学报:医学版,2014,52(4):97-106.

[8]NOMURA F,YAGUCHI M,TOGAWA A,et al.Enhancement of polyadenosine diphosphate ribosylation in human hepatocellular carcinoma[J].Gastroenterol Hepatol,2000,15(7):529-535.

[9]FREDERICK K,MORITZVON W,ALBRECHT S,et al.High nuclear poly-(ADP-ribose)-polymerase expression is prognostic of improved survival in pancreatic cancer[J].Histopathology,2012,61(6):409-416.

[10]XIE QX,LIN XC,HAN CX,et al.Significance of Caspase-3 expression in renal cell cancer[J].China Med Eng,2005,13(5):486-488.(in Chinese)谢庆祥,林吓聪,韩聪祥,等.Caspase-3在肾细胞癌中的表达及其意义[J].中国医学工程,2005,13(5):486-488.

[11]WINTER NR,KRAMER A.Loss of Caspase-1 and Caspase-3 protein expression in human prostate cancer[J].Cancer Res,2001,61(10):1277-1232.

[12]WANG CY,MENG LX,DING ZJ.Expression of PTEN,Survivin and Caspase-3 in human esophageal squmous carcinoma[J].J Mod Oncol,2015,23(4):493-496.(in Chinese)王传艳,孟令新,丁兆军.PTEN、Survivin和Caspase-3在食管鳞癌中的表达[J].现代肿瘤医学,2015,23(4):493-496.

[13]LONG H,WU QM,MA SL,et al.Expression of Midkine and Caspase-3 and their relationship with apoptosis in pancreatic cancer[J].World Chin J Dig,2008,16(35):4015-4019.(in Chinese)龙辉,吴清明,马松林,等.Midkine和Caspase-3在胰腺癌中的表达与细胞凋亡的关系[J].世界华人消化杂志,2008,16(35):4015-4019.

[14]DU Y,FENG YZ,LI F.Expression of livin and caspase-3 and their clinical significance in pancreatic carcinoma[J].Chin J Clin Exp Pathol,2009,25(6):610-614.(in Chinese)杜缘,冯一中,李峰.胰腺癌中livin、caspase-3的表达及其临床意义[J].临床与实验病理学杂志,2009,25(6):610-614.

[15]KOH DW,DAWSON TM,DAWSON VL.Mediation of cell death by poly(ADP-ribose)polymerase-1[J].Pharmacol Res,2005,52(1):5-14.

[16]SUI WY,CHEN CY,LUO ZY,et al.The expression of PAPR-1 and Caspase-3 in human breast cancer and their clinical significance[J].J Univ Sourth China:Med Edit,2010,38(2):210-213.(in Chinese)眭文妍,陈春燕,罗招阳,等.PARP-1和Caspase-3在乳腺癌组织中的表达及临床意义[J].南华大学学报:医学版,2010,38(2):210-213.

[17]GAO J,CHEN HL.Caspase-3,PARP proteins expression and their significances in human lung cancer[J].J Mathematical Med,2006,19(1):34-36.(in Chinese)高俊,陈洪雷.Caspase-3、PARP蛋白在肺癌组织中的表达及临床意义[J].数理医药学杂志,2006,19(1):34-36.

[18]QI SQ,DE S,LI DM,et al.Expressions of PARP-1,Caspase-3,survivin and Bax in cervical squamous cell carcinoma tissues and their clinical significances[J].J Jilin Univ:Med Edit,2013,39(3):512-516.(in Chinese)齐斯琴,德胜,李冬梅,等.子宫颈鳞癌组织中PARP-1、Caspase-3、survivin和Bax的表达及其临床意义[J].吉林大学学报:医学版,2013,39(3):512-516.

[19]HU SM,ZHANG SD,HAO YP,et al.Expressions and significance of PARP-1 and Caspase-3 in colorectal neoplasms[J].Chin J Gastroenterol,2009,14(2):83-87.(in Chinese)胡顺明,张世栋,郝艳萍,等.PARP-1和Caspase-3在结直肠肿瘤中的表达和意义[J].胃肠病学,2009,14(2):83-87.

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