Monoclonal antibodies( m Abs) have a high specificity and have been widely used in various diseases,especially in the treatment and study of liver injury induced by viruses. In recent years,human m Abs directly prepared from single B lymphocytes have been getting more and more attention due to high antigenic specificity,low immunogenicity,and good safety and efficiency. With the continuous improvement in monoclonal antibody technology and polymerase chain reaction technology,the antibody preparation technology based on single B lymphocytes has been developed greatly. Although there are rare reports about the m Abs which are derived from single B lymphocytes and applied in liver diseases,the application of such m Abs is promising in the treatment and study of liver diseases. This article reviews the research advances in the development of antibody preparation technology based on single B lymphocytes and the application of the prepared antibodies in liver diseases.