Objective To analyze the clinical efficacy of glucocorticoid therapy in addition to conventional treatment for patients with drug-induced cholestatic liver disease. Methods A total of 115 patients with drug- induced cholestatic liver disease who were admitted to Affiliated Hospital of Inner Mongolia University for the Nationalities from January 2010 to December 2014 were collected and divided into glucocorticoid treatment group and non- glucocorticoid treatment group. The glucocorticoid treatment group was given methylprednisolone sodium succinate 120 mg once daily by intravenous injection in addition to conventional treatment. The indicator for glucocorticoid response was defined as 10% decrease of total bilirubin( TBil) on the third day or 30% decrease on the seventh day. Then the patients were orally given prednisone tablets 10 mg three times daily based on the level of TBil,and the administration of prednisone tablets was adjusted to twice daily a week later. The course of treatment was less than three weeks. Comparison of continuous data in normal distribution between the two groups was made by t test,and comparison of continuous data not in normal distribution between the two groups was made by rank sum test. Results The levels of gamma- glutamyl transpeptidase( GGT),alanine aminotransferase( ALT),and TBil in the glucocorticoid treatment group decreased significantly on days 3,7,and 14 of treatment compared with those before treatment( tGGT= 3. 64,13. 08,16. 22; tALT= 2. 39,4. 73,8. 36; tTBil= 3. 46,7. 41,13. 17; all P < 0. 05). Compared with the non- glucocorticoid treatment group,the glucocorticoid treatment group had significantly lower AST and ALT levels before treatment and on days 3 and 7 of treatment( all P > 0. 05). The GGT,AST,and TBil levels in the glucocorticoid treatment group were significantly lower than those in the non- glucocorticoid treatment group on day 14 of treatment( t = 7. 074,2. 929,2. 018; all P < 0. 05). The average decreasing speed of bilirubin in the glucocorticoid treatment group was12. 21% ± 2. 91% per day,significantly faster than 6. 27% ± 0. 92% per day in the non- glucocorticoid treatment group( t = 15. 11,P <0. 001). Conclusion Glucocorticoid therapy in addition to conventional treatment for patients with drug- induced cholestatic liver disease is superior to conventional treatment alone in lowering TBil,GGT,and ALT levels.