Objective To study the relationship between Epstein-Barr virus(EBV) DNA and EBV antibody levels.Methods A retrospective study was conducted on 143 patients who underwent EBV DNA testing in serum and lymphocytes from January to August,2014.The clinical data,including age,sex,blood routine and biochemical examination,serum / lymphocyte EBV DNA,EBV-VCA-Ig M,and EBV-VCA-Ig G were recorded.Spearman correlation analysis was used to measure the degree of the relationships.Kruskal-Wallis H test or Mann-Whitney U test were applied to compare the differences between three or two groups.Results The spearman rank correlation coefficient between serum EBV DNA and EBV-VCA-Ig M levels was 0.434(P = 0.00),while the correlation coefficients of serum EBV DNA level and EBV-VCA-Ig M level with lymphocyte EBV DNA,alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),gamma-glutamyl transpeptidase(GGT),white blood cells(WBC),and heterotypic lymphocytes ranged from0.169 to 0.693(P < 0.05) and from 0.153 to 0.434(P < 0.05),respectively.The spearman rank correlation coefficient of lymphocyte EBV DNA with serum EBV DNA,EBV-VCA-Ig M,AST,ALP,WBC,and heterotypic lymphocytes ranged from 0.207 to 0.693(P <0.05).The positive rates of EBV DNA in lymphocytes and serum were 70.6%(101 /143) and 32.2%(46 /143),respectively.The EBV-VCA-Ig M positive rate was 22.4%(32 /143),and 22.5%(25 /111) of the patients with negative EBV-VCA-Ig M were positive for serum EBV DNA.The overall positive rate for EBV-VCA-Ig G was 91.6%(131 /143).The EBV-VCA-Ig M positive rate in patients with positive serum EBV DNA(21 /46,45.7%) was significantly higher than that in patients with positive lymphocyte EBV DNA(29 /101,28.7%)(χ2= 7.95,P < 0.01).Conclusion Serum EBV DNA level is strongly correlated with EBV-VCA-Ig M level.Moreover,serum EBV DNA exhibits a higher positive rate than EBV-VCA-Ig M.Simultaneous detection of serum EBV DNA and EBV-VCA-Ig M may benefit the diagnosis of current EBV infection.