Abstract: Objective To investigate the levels of molecular markers of prethrombotic state in liver cirrhosis( LC) patients with portal vein thrombosis( PVT) and their significance. Methods Seventy- two LC patients who were admitted to our hospital from 2011 to 2013 were selected for analysis. Among the patients,thirty- two were diagnosed with PVT by computed tomography. The plasma levels of granular membrane protein- 140( GMP- 140),von Willebrand Factor antigen( v WF: Ag),thrombomodulin( TM),and D- dimer( DD) were measured by enzyme- linked immunosorbent assay. The data were analyzed using SPSS 11. 0. Continuous data were expressed as mean ±standard deviation and compared between the two groups by independent- samples t test. Results The concentrations of GMP- 140,TM,v WF: Ag,and DD in patients with PVT were significantly higher than those in patients without PVT( 20. 68 ± 1. 49 vs 13. 05 ± 0. 97 μg / L,P < 0. 05; 47. 24 ± 1. 36 vs 34. 05 ± 5. 03 μg / L,P < 0. 05; 194. 32% ± 7. 68% vs 136. 21% ± 3. 68%,P < 0. 05; 0. 86 ± 0. 12 vs 0. 42 ±0. 08 mg / L,P < 0. 05). The plasma levels of GMP- 140,TM,v WF: Ag,and DD in PVT patients with severe esophageal varices were significantly higher than those in patients with mild esophageal varices( 19. 68 ± 1. 29 vs 12. 05 ± 1. 07 μg / L,P < 0. 05; 45. 24 ± 1. 26 vs35. 05 ± 4. 83 μg / L,P < 0. 05; 196. 32% ± 6. 68% vs 141. 21% ± 3. 45 %,P < 0. 05; 0. 79 ± 0. 12 vs 0. 36 ± 0. 08 mg / L,P < 0. 05).The plasma levels of GMP- 140,TM,v WF: Ag,and DD in PVT patients with gastrointestinal bleeding were significantly higher than those in patients without gastrointestinal bleeding( 18. 98 ± 1. 18 vs 11. 98 ± 1. 12 μg / L,P < 0. 01; 46. 78 ± 1. 35 vs 36. 02 ± 4. 78 μg / L,P <0. 01; 197. 32% ± 6. 39% vs 138. 21% ± 4. 12 %,P < 0. 01; 0. 81 ± 0. 14 vs 0. 35 ± 0. 12 mg / L,P < 0. 01). Conclusion The levels of molecular markers of prethrombotic state can be used to monitor PVT in LC patients.