Objective To evaluate the significance of combined analysis of different autoantibodies in the diagnosis of primary biliary cirrhosis( PBC). Methods Two- hundred and forty- five patients with liver diseases were randomly selected. According to the disease type,patients were divided into PBC group( n = 162),autoimmune hepatitis( AIH) group( n = 42),and liver disease control( LDC) group( n = 41). ELISA assay was used to detect serum anti- mitochondrial antibody( AMA- M2) and antibodies against SP100 and GP210.Western blot assay was employed to detect serum anti- SLA antibodies. Indirect immunofluorescence assay was performed to detect serum AMA. Comparison of antibody positive rates between groups was carried out using the chi- square test and Fisher' s test. Results Serum AMA,AMA- M2,and anti- GP210 positive rates of the PBC group were 93. 21%,87. 65%,and 19. 75%,respectively,significantly higher than those of the AIH group,i. e.,19. 05%,7. 14%,and 9. 52%,respectively( χ2= 97. 311,P = 0. 001; χ2= 98. 264,P =0. 001; χ2= 10. 312,P = 0. 012). The AMA,AMA- M2,and anti- GP210 positive rates of the PBC group were also significantly higher than those of the LDC group,i. e.,9. 76%,2. 44%,and 7. 32%,respectively( χ2= 142. 745,P = 0. 003; χ2= 112. 574,P = 0. 002; χ2= 15. 217,P = 0. 042). Conclusion Serum AMA has a remarkable meaning to PBC diagnosis,anti- SP100 gives a hint of PBC progression,and anti- GP210 plays a role in the diagnosis of AMA- negative PBC.