丹酚酸B对非酒精性脂肪性肝炎大鼠肝细胞凋亡的影响

王迎春; 孔维宗; 金青梅; 陈娟

doi:10.3969/j.issn.1001-5256.2014.12.022

丹酚酸B对非酒精性脂肪性肝炎大鼠肝细胞凋亡的影响

DOI: 10.3969/j.issn.1001-5256.2014.12.022

大连大学附属中山医院消化内科

基金项目:

大连市医学卫生科学研究计划项目(200967)；

详细信息

中图分类号: R575.5
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出版历程
- 收稿日期: 2014-04-24
- 出版日期: 2014-12-20

Effect of salvianolic acid B on hepatocyte apoptosis in rats with nonalcoholic steatohepatitis

Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University

Research funding:

摘要

摘要:
目的探讨丹酚酸B对非酒精性脂肪性肝炎（NASH）大鼠肝细胞凋亡的影响及对NASH的治疗作用。方法清洁级雄性SD大鼠60只,随机分为对照组、NASH模型组、丹酚酸B治疗组,每组20只。对照组以普通饲料喂养,其余2组以高脂饲料连续喂养12周复制NASH模型。第13周开始治疗组每天以浓度为1 mg/ml的丹酚酸B溶液20 ml/kg灌胃,模型组以20 ml/kg蒸馏水灌胃。治疗12周后,处死大鼠,取血及肝组织,计算肝指数,检测血清ALT、AST、甘油三酯（TG）、总胆固醇（TC）,观察肝组织病理学变化,免疫组织化学法检测肝组织细胞色素C（Cyt C）、caspase-3蛋白的表达,逆转录-聚合酶链反应（RT-PCR）检测肝组织p53、Bax、Bcl-2 mRNA的表达。结果模型组大鼠肝指数、ALT、AST、TG、TC较对照组增高,肝组织炎症明显;Cyt C及caspase-3蛋白表达增加（P值均<0.01）;Bcl-2 mRNA表达降低,p53、Bax mRNA表达增高（P值均<0.01）。治疗组与模型组相比肝指数、ALT、AST、TG、TC降低,炎症减轻;Cyt C及caspa...
- 脂肪肝 /
- 肝炎 /
- 丹酚酸B /
- 细胞凋亡 /
- 大鼠,Sprague-Dawley
Abstract:
Objective To investigate the effect of salvianolic acid B on hepatocyte apoptosis in rats with nonalcoholic steatohepatitis (NASH) and the efficacy of salvianolic acid B in the treatment of NASH. Methods Sixty clean male SD rats were randomly divided into control group, NASH model group, and salvianolic acid B treatment group, with 20 rats in each group. The control group was fed with normal diet, while the other two groups with high-fat diet for 12 continuous weeks to establish a NASH model. From week 13, rats in the treatment group were gavaged with 1 mg / ml salvianolic acid B (20 ml / kg b. w. daily) ; while the model group with distilled water (20 ml / kg b. w daily) . After 12 weeks of treatment, the rats were sacrificed; blood and hepatic tissues were sampled, liver index (liver weight / body weight) calculated, serum levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , triglycerides (TG) , and total cholesterol (TC) measured, and the pathologic changes in hepatic tissues observed. The protein expression of caspase-3 and cytochrome C (Cyt C) in hepatic tissues was measured by immunohistochemical methods. The mRNA expression of p53, Bax, and Bcl-2 in hepatic tissues was measured by RT-PCR. Results Compared with the normal group, the model group showed higher liver index and serum levels of ALT, AST, TG, and TC and more obvious inflammation of hepatic tissues; the protein expression of Cyt C and caspase-3 was significantly increased (P < 0. 01 for both) ; the mRNA expression of Bcl-2 was significantly reduced (P < 0. 01) , while that of Bax and p53 was significantly increased (P < 0. 01 for both) . Compared with the model group, the treatment group showed alleviated inflammation and reduced liver index and serum levels of ALT, AST, TG, and TC; the protein expression of Cyt C and caspase-3 was significantly reduced (P < 0. 01 for both) ; Bcl-2 mRNA expression was significantly increased (P < 0. 05) , p53 mRNA expression significantly reduced (P < 0. 05) , and Bax mRNA expression also significantly reduced (P < 0. 01) . Conclusion Salvianolic acid B can inhibit hepatocyte apoptosis and treat NASH by regulating the mRNA expression of Bcl-2, p53, and Bax and reducing the protein expression of Cyt C and caspase-3.
- fatly liver /
- hepatitis /
- Sal B /
- apoptosis /
- rats, Sprague-Dawley

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