Objective To investigate the value and clinical application of posterior- anterior cystohepatic triangle dissection in laparoscopic cholecystectomy (LC) . Methods The medical records of 250 patients who underwent LC were reviewed. The clinical data, including operating time, amount of bleeding, and surgical complications, were retrospectively analyzed. Results LC was successfully completed in 246 cases. The operating time ranged from 25 min to 120 min, with a mean of 42 min. The amount of bleeding ranged from 5 ml to 80 ml, with a mean of 20 ml. Two cases were converted to open surgery due to gastric carcinoma or cholecystocolonic fistula, and 2 cases underwent laparoscopic common bile duct exploration. There was no report of severe complications in the entire group, such as abdominal hemorrhage, bile leakage, and biliary stricture. Conclusion With proper identification of the anatomical structure, it is safe and feasible to perform LC by dissection of the posterior- anterior cystohepatic triangle, which effectively helps avoid hemorrhage and bile duct injury during operation.