硫代乙酰胺诱导的A型肝性脑病小鼠焦虑样反应观察

陈启明; 袁宏; 戴丽蓉; 蒋妮; 任文婷; 陈青锋

doi:10.3969/j.issn.1001-5256.2014.10.024

硫代乙酰胺诱导的A型肝性脑病小鼠焦虑样反应观察

DOI: 10.3969/j.issn.1001-5256.2014.10.024

1. 兰州大学第一医院
2. 兰州大学基础医学院

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中图分类号: R747.9
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出版历程
- 收稿日期: 2014-02-15
- 出版日期: 2014-10-20

Anxiety in mice with acute hepatic encephalopathy induced by thioacetamide

The First Hospital of Lanzhou University

摘要

摘要:
目的探讨硫代乙酰胺（TAA）诱导的A型肝性脑病小鼠的精神障碍。方法 30只雄性昆明种小鼠随机分为实验组15只,对照组15只。实验组采用TAA 200 mg·kg-1·d-1,连续腹腔注射3 d至累积计量到600 mg/kg,正常对照组腹腔注射生理盐水,造模24 h后进行脑功能评分、明暗箱、旷场及高架十字实验。行为学检测结束后24 h行血氨及肝脏生化指标检测。两组间比较采用独立样本t检验。结果实验组小鼠在明暗箱实验中的明箱滞留时间（t=-4.006,P<0.01）与穿梭次数（t=-2.656,P<0.05）;旷场实验的中央活动路程（t=-3.639,P<0.05）、中央活动时间（t=-2.294,P<0.05）、垂直运动次数（t=-2.282,P<0.05）、理毛时间（t=5.992,P<0.01）;高架十字实验的开臂进入次数（t=-3.584,P<0.05）、开臂滞留时间（t=-3.992,P<0.05）与对照组小鼠相比,差异均具有统计学意义。但旷场实验中两组小鼠活动总路程差异无统计学意义（t=-0.96,p>0.05）。且实验组小...
- 肝性脑病 /
- 焦虑 /
- 硫代乙酰胺
Abstract:
Objective To evaluate the mental disorders in mice with thioacetamide ( TAA) -induced acute hepatic encephalopathy ( AHE) .Methods Thirty male Kunming mice were equally and randomly divided into two groups ( treatment group and control group) . Mice in the treatment group received an intraperitoneal injection of TAA ( 200 mg·kg-1·d-1for 3 days, up to 600 mg·kg-1) , as compared with the same volume of physiological saline in the control group. Neurological function score, light /dark box, open field, and elevated plus-maze test were used to determine behavioral parameters at 24 h after the model was established. Serum ammonia, aspartate amino transferase ( AST) , alanine aminotransferase ( ALT) , and total bilirubin ( TBil) were measured at 24 h post determination of behavioral parameters. Comparison between two groups was made by independent-samples t test. Results In the light /dark box test, the treatment group had significantly reduced residence time in the light box and number of shuttles between two boxes ( t =-4. 006, P < 0. 01; t =-2. 656, P < 0. 05) ; in the open field test, the treatment group had significantly reduced central distance, central time, and number of vertical movements ( t =-3. 639, P < 0. 05;t =-2. 294, P < 0. 05; t =-2. 282, P < 0. 05) , as well as increased grooming time ( t = 5. 992, P < 0. 01) ; meanwhile, the number of open-arm entries and residence time in the open arm for the treatment group were also reduced in the elevated plus-maze test, as compared with the control group ( t =-3. 584, P < 0. 05; t =-3. 992, P < 0. 05) . However, the total distance in the open field test showed no significant difference between the two groups ( t =-0. 96, P > 0. 05) . Moreover, compared with the control group, the treatment group had significantly higher levels of serum ammonia ( t =-3. 168, P < 0. 05) , ALT ( t = 4. 316, P < 0. 05) , AST ( t =-2. 581, P < 0. 05) , and TBil ( t =-9. 127, P < 0. 01) . Conclusion Mice with AHE induced by an intraperitoneal injection of low-dose TAA have anxiety-related behaviors; the anxiety-related behaviors are not associated with reduced locomotor activities.
- hepatic encephalopathy /
- anxiety /
- thioacetamide

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