雌激素受体α-29位基因多态性与甘肃地区汉族HBV相关慢加急性肝衰竭患者的关系

周宁; 张月荣; 魏世芳; 吴丽阳; 李象霖; 孙美英

doi:10.3969/j.issn.1001-5256.2014.10.008

雌激素受体α-29位基因多态性与甘肃地区汉族HBV相关慢加急性肝衰竭患者的关系

DOI: 10.3969/j.issn.1001-5256.2014.10.008

兰州市第一人民医院感染科

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中图分类号: R575.3
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出版历程
- 收稿日期: 2014-02-20
- 出版日期: 2014-10-20

Association of ERα gene polymorphism at position 29 with HBV-related acute-on-chronic liver failure

Department of Infectious Diseases, The First People's Hospital of Lanzhou

摘要

摘要:
目的了解雌激素受体（ER）α-29位多态性与HBV感染后果的关系,从基因水平上探讨HBV相关慢加急性肝衰竭（HBV-ACLF）的发病机制。方法收集2011年8月至2013年11月在兰州市第一人民医院住院及门诊就诊的甘肃籍汉族患者,采用聚合酶链反应-限制性片段长度多态性法检测61例HBV-ACLF患者、79例慢性乙型肝炎（CHB）患者、69例HBV感染自愈者的ERα-29位多态性。用基因计数法计算检验人群的等位基因频率,进行Hardy-Weinberg遗传平衡定律检验。应用非条件Logistic回归分析将混杂因素进行调整。基因型及等位基因频率比较采用行×列的χ2检验。结果 HBV-ACLF组TT基因型和T等位基因频率（55.7%,77.1%）明显高于CHB组患者（31.6%,54.4%）,差异有统计学意义（χ2=8.194,P=0.004;χ2=15.341,P<0.001）,携带T等位基因HBV感染重症化的风险是C等位基因的2.811倍（OR=2.811,95%CI:1.6624.753）。HBV-ACLF组TT基因型和T等位基因频率（55.7%,77.1%...
- 肝功能衰竭 /
- 受体,雌激素 /
- 多态性,单核苷酸 /
- 多态性,限制性片段长度
Abstract:
Objective To investigate the relationship between estrogen receptor alpha ( ERα) gene polymorphism at position 29 and hepatitis B virus ( HBV) -related acute-on-chronic liver failure ( HBV-ACLF) and the genetic pathogenesis of HBV-ACLF. Methods A total of 61HBV-ACLF patients, 79 patients with chronic hepatitis B ( CHB) , and 69 patients who automatically recovered from HBV infection were included in the study. The ERα gene polymorphism at position 29 was analyzed by polymerase chain reaction-restriction fragment length polymorphism. The allele frequency was calculated by gene counting, and Hardy-Weinberg equilibrium test was performed. Confounding factors were adjusted by unconditional logistic regression analysis. Comparisons of genotype frequency and allele frequency were made by chi-square test ( R × C) . Results The frequencies of TT genotype and T allele of ERα gene at position 29 were significantly higher in HBV-ACLF patients than in CHB patients (55.7% vs 31.6%, χ2=8. 194, P =0. 004; 77. 1% vs 54. 4%, χ2=15. 341, P <0. 001) , and the risk of worsening in HBV infection patients with T allele was 2. 811 times that in HBV infection patient with C allele ( OR =2. 811, 95% CI: 1. 662-4. 753) . The frequencies of TT genotype and T allele of ERα gene at position 29 were significantly higher in HBV-ACLF patients than in patients who recovered from HBV infection ( 55. 7% vs11. 6%, χ2=28. 849, P <0. 001; 77. 1% vs 32. 6%, χ2=51. 401, P <0. 001) , and the risk of worsening in HBV infection patients with T allele was 6. 938 times that in patients with C allele ( OR =6. 938, 95% CI: 3. 995-12. 050) . Conclusion TT genotype and T allele of ERα gene at position 29 might be the susceptibility gene for HBV-ACLF.
- liver failure /
- receptor, estrogen /
- polymorphisms, single nucleotide /
- polymorphisms, restriction fragment length

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