不同病毒载量HBV感染者外周血T淋巴细胞亚群和转化生长因子β的变化及意义

李彩东; 吴斌; 陈锡莲; 田鹏飞; 段正军

doi:10.3969/j.issn.1001-5256.2014.09.011

不同病毒载量HBV感染者外周血T淋巴细胞亚群和转化生长因子β的变化及意义

DOI: 10.3969/j.issn.1001-5256.2014.09.011

甘肃省兰州市第二人民医院肝病研究所

基金项目:

兰州市科技发展计划项目(2012-1-12)；

详细信息

中图分类号: R512.62
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出版历程
- 收稿日期: 2014-04-16
- 出版日期: 2014-09-20

Changes in peripheral blood T cell subsets and TGFβ and their clinical significance among HBV infection patients with different viral loads

Institute of Hepatology, The Second People's Hospital of Lanzhou

Research funding:

摘要

摘要:
目的通过检测不同病毒载量慢性乙型肝炎（CHB）患者和HBV携带者（ASC）外周血T淋巴细胞各亚群变化的规律,探讨T淋巴细胞与人转化生长因子（TGF）β、ALT及TBil相关性。方法选择2012年7月至2012年12月兰州市第二人民医院收治的175例HBV感染者作为研究对象,分为ASC组（112例）、CHB组（63例）,以同期健康体检者84例作为对照组。运用流式细胞术检测3组血清中T淋巴细胞亚群水平,同时检测肝功能指标等。正态分布的计量资料多组间比较采用方差分析,偏态分布的计量资料组间比较采Kruskal-Wallis H秩和检验。结果与健康对照组相比,ASC和CHB组TGFβ表达水平显著升高（P均<0.05）,CD3+、CD4+T淋巴细胞百分数显著降低（P<0.05或P<0.01）,CD4+与CD8+T淋巴细胞比值显著降低（P<0.01）,CD8+T淋巴细胞百分数显著升高（P<0.05或P<0.01）。ASC与CHB组HBeAg阳性组和HBeAg阴性组患者CD3+、CD4+T淋巴细胞百分数均降低,CD8+T淋巴细胞百分数升高,CD4+与CD8+T淋巴...
- 肝炎,乙型,慢性 /
- T淋巴细胞 /
- 转化生长因子β
Abstract:
Objective To study the changes in peripheral blood T cell subsets among chronic hepatitis B ( CHB) patients and asymptomatic hepatitis B virus ( HBV) carriers ( ASC) , who have different viral loads, and to investigate the correlation of T cell subsets with transforming growth factor beta ( TGFβ) , alanine aminotransferase, and total bilirubin. Methods A total of 175 HBV infection patients admitted to our hospital from July to December, 2012 were recruited and divided into ASC group ( n = 112) and CHB group ( n = 63) ; 84 healthy controls who underwent physical examination in the same period were selected as control group. The percentages of T cell subsets in serum were determined by flow cytometry, and liver function parameters were measured. For normally distributed continuous data, comparison between groups was made by analysis of variance; for non- normally distributed continuous data, comparison between groups was made by Kruskal- Wallis H rank sum test. Results Among the 175 HBV infection patients, the percentages of CD3+, CD4+, and CD8+T cells and CD4+/CD8+ratio were72. 14%- 74. 07%, 38. 43%- 39. 47%, 30. 74%- 31. 42%, and 1. 31- 1. 34, respectively. Compared with the control group, the ASC group and CHB group had significantly increased TGFβ levels ( P < 0. 05 for both) , significantly reduced percentages of CD3+and CD4+T cells ( P < 0. 05 or P < 0. 01) , significantly reduced CD4+/CD8+ratios ( P < 0. 01) , and significantly increased percentages of CD8+T cells ( P<0.05 or P <0.01) .="" in="" the="" asc="" and="" chb="" both="" hbeag-="" positive="" negative="" patients="" had="" reduced="" percentages="" of="" t="" increased="" ratios.="" no="" correlation="" was="" found="" between="" hbv="" dna="" loads="" cell="" subsets="" two="" groups="" p="">0. 05) . Conclusion TGFβ may be involved in the pathogenesis of CHB, and its immunosuppressive effect may be exerted by inhibiting T cells and antigen- presenting cell maturation.
- hepatitis B, chronic /
- T-lymphocytes /
- transforming growth factor beta

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