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Toll样受体2介导的Th17细胞活化在HBV感染中的作用

赵荣荣 杨晓飞 董杰 黄长形 张野 连建奇

引用本文:
Citation:

Toll样受体2介导的Th17细胞活化在HBV感染中的作用

DOI: 10.3969/j.issn.1001-5256.2014.09.010
基金项目: 

陕西省自然科学基础研究计划项目(2012JQ4023); 国家“十二五”传染病重大专项(2012ZX10002-007-001-006); 

详细信息
  • 中图分类号: R512.62

Toll- like receptor 2 drives Th17 cell activation in inflammatory response to hepatitis B virus infection

Research funding: 

 

  • 摘要:

    目的探讨乙型肝炎患者外周血单个核细胞(PBMC)中Toll样受体2(TLR2)与Th17细胞的相关性,为阐述HBV感染诱导炎症应答机制提供理论和实验依据。方法选取2012年7月-2013年7月唐都医院感染科门诊和住院的34例乙型肝炎初治患者,其中24例慢性乙型肝炎和10例急性乙型肝炎;另外选取健康对照者10例,分离PBMC,利用HBV C基因型Envelope区肽段(特异性)或佛波酯联合伊屋诺霉素(非特异性)刺激,流式细胞术检测TLR2表达及Th17细胞百分比。进一步用TLR2的激动剂刺激PBMC,检测Th17细胞变化情况。组间比较采用Kruskal-Wallis H检验。结果在非特异性刺激条件下,Th17细胞在慢性乙型肝炎患者体内的百分比(4.08±1.78)%显著高于急性乙型肝炎患者(1.85±1.28)%及健康对照者(2.09±0.53)%(P=0.000 9、0.000 4),而TLR2+及IL-17A+TLR2+的表达在急、慢性乙型肝炎患者与健康人外周血中差异均无统计学意义(P均>0.05)。在特异性刺激条件下Th17及TLR2的表达在慢性乙型肝炎患者体内的表达显著高于...

     

  • [1] GANEM D, PRINCE AM.Hepatitis B virus infection-natural history and clinical consequences[J].N Engl J Med, 2004, 350 (11) :1118-1129.
    [2] CHISARI FV, ISOGAWA M, WIELAND SF.Pathogenesis of hepatitis B virus infection[J].Pathol Biol (Paris) , 2010, 58 (4) :258-266.
    [3] O'INN DB, PALMER MT, LEE YK, et al.Emergence of the Th17pathway and its role in host defense[J].Adv Immunol, 2008, 99:115-163.
    [4]ZHANG JY, ZHANG Z, LIN F, et al.Interleukin-17-producing CD4+T cells increase with severity of liver damage in patients with chronic hepatitis B[J].Hepatology, 2010, 51 (1) :81-91.
    [5] WU W, LI J, CHEN F, et al.Circulating Th17 cells frequency is associated with the disease progression in HBV infected patients[J].J Gastroenterol Hepatol, 2010, 25 (4) :750-757.
    [6]AKIRA S, TAKEDA K.Toll-like receptor signaling[J].Nat Rev Immunol, 2004, 4 (7) :499-511.
    [7]WU J, LU M, MENG Z, et al.Toll-like receptor-mediated control of HBV replication by nonp arenchymal liver cells in mice[J].Hepatology, 2007, 46 (6) :1769-1778.
    [8] ZHANG Y, LIAN JQ, HUANG CX, et al.Overexpression of Toll-like receptor 2/4 on monocytes modulates the activities of CD4+CD25+regulatory T cells in chronic hepatitis B virus infection[J].Virology, 2010, 397 (1) :34-42.
    [9] ZHANG Y, COBLEIGH MA, LIAN JQ, et al.A proinflammatory role for interleukin-22 in the immune response to hepatitis B virus[J].Gastroenterology, 2011, 141 (5) :1897-1906.
    [10]Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association.The guideline of prevention and treatment for chronic hepatitis B (2010 version) [J].J Clin Hepatol, 2011, 27 (1) :Ⅰ-ⅩⅥ. (in Chinese) 中华医学会肝病学分会、感染病分会.慢性乙型肝炎防治指南 (2010年版) [J].临床肝胆病杂志, 2011, 27 (1) :Ⅰ-ⅩⅥ.
    [11]LIN ZZ, YU XP, SU ZJ.Detection of the ratios of Treg/Th17 and study on its correlation with liver function in peripheral blood of patients with chronic hepatitis B[J].Chin J Immunol, 2012, 28 (11) :1036-1040. (in Chinese) 林振忠, 朱雪平, 苏智军, 等.慢性乙型肝炎患者外周血Treg/Th17比率的检测及其与肝功能的相关性研究[J].中国免疫学杂志, 2012, 28 (11) :1036-1040.
    [12]DURAISINGHAM SS, HORNIG J, GOTCH F, et al.TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Th17 polarization of naive T cells but do stimulate Th1 and Th17 memory responses[J].J Immunol, 2009, 183 (4) :2242-2251.
    [13] REYNOLDS JM, PAPPU BP, PENG J, et al.Toll-like receptor2signaling in CD4 (+) T lymphocytes promotes T helper 17 responses and regulates the pathogenesis of autoimmune disease[J].Immunity, 2010, 32 (5) :692-702.
    [14]CHEN X, ZHANG M, ZHU X, et al.Engagement of Toll-like receptor 2 on CD4+T cells facilitates local immune responses in patients with tuberculo pleurisy[J].J Infect Dis, 2009, 200 (3) :399-408.引证本文:ZHAO RR, YANG XF, DONG J, et al.Toll-like receptor 2 drives Th17 cell activation in inflammatory response to hepatitis B virus infection[J].J Clin Hepatol, 2014, 30 (9) :877-881. (in Chinese) 赵荣荣, 杨晓飞, 董杰, 等.Toll样受体2介导的Th17细胞活化在HBV感染中的作用[J].临床肝胆病杂志, 2014, 30 (9) :877-881.
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  • 收稿日期:  2014-06-30
  • 出版日期:  2014-09-20
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