13C-美沙西丁呼气试验和吲哚氰绿清除试验对急性肝损害大鼠肝功能评价的比较

毛静怡; 李鑫; 王晨; 徐有青

doi:10.3969/j.issn.1001-5256.2013.11.016

13C-美沙西丁呼气试验和吲哚氰绿清除试验对急性肝损害大鼠肝功能评价的比较

DOI: 10.3969/j.issn.1001-5256.2013.11.016

1. 北京垂杨柳医院消化内科
2. 首都医科大学附属北京天坛医院消化内科

基金项目:

吴阶平医学基金会肝病医学部肝病实验诊断研究基金(LDWMF-PJ-2011B001)；

详细信息

中图分类号: R575.2
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出版历程
- 收稿日期: 2013-03-20
- 出版日期: 2013-11-20

Roles of indocyanine green clearance test and 13C- methacetin breath test in assessing liver function in acute liver injury: a comparative study

Department of Gastroenterology, Beijing Chuiyangliu Hospital

Research funding:

摘要

摘要: 目的对比研究13C-美沙西丁呼气试验（13C-MBT）及吲哚氰绿清除试验在急性肝损害肝功能评估中的价值。方法 48只SD大鼠随机分为对照组和模型组,对照组给予腹腔注射生理盐水;模型组分为6、12、24、48、72 h组,腹腔注射D-氨基半乳糖诱导急性肝损害模型。观察各组肝组织病理学、吲哚氰绿15 min滞留率（ICGR15）、13C-MBT相关参数:包括13CO2峰度曲线达峰时间（T max）、代谢速率峰值（MV max）、120 min13CO2累积呼出丰度（CUM120）和呼气峰值（DOB max）的变化。组间比较采用单因素方差分析,多个样本均数两两比较采用SNK-q检验,两变量间的关系采用Pearson相关分析。结果与对照组比较,模型组各组病理学积分明显升高（P<0.05）,DOB max、MV max40和CUM120明显降低（P<0.05）。病理积分与T max呈正相关（r=0.593,P<0.05）,与MV max、CUM120和DOB max呈负相关（相关系数分别为-0.731、-0.618、-0.592,P<0.01）。与对照组比较,12、24、...
- 呼吸试验 /
- 吲哚花青绿 /
- 肝功能不全 /
- 半乳糖胺
Abstract: Objective To conduct a comparative study of indocyanine green ( ICG) clearance test and13C- methacetin breath test (13C- MBT) in assessing the liver function in acute liver injury. Methods Forty- eight male Sprague- Dawley rats were randomly divided into control group and 6, 12, 24, 48, and 72 h model groups. The rats in control group were intraperitoneally injected with saline, while the rats in model groups were intraperitoneally injected with D- galactosamine ( 450 mg / kg) to establish an acute liver injury model. The pathological score of liver tissue, ICG retention rate at 15 minutes ( ICG R15) , and parameters of13C- MBT, such as duration to peak of13CO2delta over baseline ( DOB) curve ( T max) , maximum metabolization velocity ( MV max) , cumulative excretion at 120 min ( CUM120) , and maximum DOB ( DOB max) were monitored.Comparison between groups was made by one- way analysis of variance; pairwise comparison of means between samples was made by SNK- q test;correlation between two variables was analyzed by Pearson's correlation test. Results Compared with the control group, all model groups showed significantly increased pathological scores ( P < 0. 05) and significantly decreased MV max, CUM120, and DOB max ( P <0.05) . Pathological score was positively correlated with T max ( r =0.593, P <0.05) and negatively correlated with MV max, CUM120, and DOB max ( r =-0.731, -0.618, and-0.592, P < 0. 05 for all) . Compared with the control group, the 12, 24, and 48 h model groups had significantly increased ICG R15 ( P < 0. 05) , but there was no significant difference in ICG R15 between the 6, 12 h model group and control group ( P > 0. 05) . Conclusion Both13C- MBT and ICG clearance test can be used as the effective tools for monitoring the liver function in acute liver injury.13C- MBT is more sensitive than ICG clearance test in the early diagnosis of acute liver injury.
- breath tests /
- indocyanine green /
- hepatic insufficiency /
- galactosamine

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参考文献(1)

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