唐山地区献血人群隐匿性乙型肝炎病毒感染分析

曹晓; 曹庆宝; 张国强; 常在娟; 李淑萍; 徐微微

唐山地区献血人群隐匿性乙型肝炎病毒感染分析

1. 唐山市中心血站
2. 唐山市第三医院

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中图分类号: R512.62
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出版历程
- 出版日期: 2013-02-20

Analysis of occult hepatitis B virus infection among blood donors in Tangshan, China

摘要

摘要:
目的了解唐山地区无偿献血人群隐匿性乙型肝炎感染情况。方法用ELISA法检测无偿献血者的乙型肝炎血清标志物,对于HBsAg阴性样本,进行HBV核酸检测（NAT）,NAT阳性样本,用罗氏试剂确证HBV DNA载量。结果共检测116 741例血样,证实隐匿性乙型肝炎感染者35例,占总献血人数的0.29‰。其中97.1%隐匿性乙型肝炎感染者样本的HBV DNA滴度低于102IU/ml。在HBV DNA阳性人群中,抗-HBc阳性率较高,占81.5%,抗-HBs阳性或乙型肝炎病毒血清标志物全阴性也可检出HBV DNA分别占55.6%和22.9%。结论唐山地区献血人群中血清HBsAg阴性者存在一定比例的隐匿性HBV感染,其HBV病毒载量均较低,核酸检测能够提高HBV感染的检出率。
- 肝炎,乙型 /
- 肝炎病毒,乙型 /
- 酶联免疫吸附测定
Abstract:
Objective To investigate the prevalence of occult hepatitis B virus (HBV) infection (OBI) among the volunteer blood donors in Tangshan, China.Methods Enzyme-linked immunosorbent assay was used to detect serum HBV markers in the blood donors.Nucleic acid test (NAT) was performed in the hepatitis B surface antigen (HBsAg) -negative blood samples to detect HBV DNA, and Roche reagent was used to measure the viral load of HBV DNA in NAT-positive samples.Results Among the HBsAg-negative blood samples of 116 741 blood donors, 39 (0.033%) had positive results in NAT, and 35 (0.029%) were confirmed with OBI.The viral load of HBV DNA was less than 102 IU/ml in 97.1% of the blood donors with OBI.Of the blood donors with OBI, 27 (77.1%) were positive for at least one of hepatitis B surface antibody (HBsAb) , hepatitis B e antigen, hepatitis B e antibody, and hepatitis B core antibody (HBcAb) , and 8 (22.9%) were negative for all serum HBV markers.Of the 27 positive blood donors, 22 (81.5%) were positive for HBcAb, and 15 (55.6%) were positive for HBsAb.Conclusion OBI occurs in some HBsAg-negative blood donors in Tangshan, and the viral load of HBV DNA is low.NAT is effective in increasing the detection rate of HBV infection.
- hepatitis B /
- hepatitis B virus /
- enzyme-linked immunosorbent assay

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参考文献(12)

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