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循环血单核细胞GPC-3mRNA和甲胎蛋白对肝癌诊断与转移监测的潜在价值

姚敏 杨燕 姚登福 卞银珠 蔚丹丹 严晓娣 陈洁

引用本文:
Citation:

循环血单核细胞GPC-3mRNA和甲胎蛋白对肝癌诊断与转移监测的潜在价值

基金项目: 

江苏省卫生科技项目(K201102); 南通市科技项目(HS2011012,K2010026); 

详细信息
  • 中图分类号: R735.7

Clinical value of circulating peripheral blood mononuclear cell glypican-3 mRNA and alpha fetoprotein for diagnosing and monitoring metastasis of hepatocellular carcinoma

Research funding: 

 

  • 摘要:

    目的分析肝癌患者外周血单核细胞中磷脂酰肌醇蛋白多糖(GPC)-3 mRNA表达及甲胎蛋白(AFP)对肝癌诊断与转移监测的价值。方法收集住院肝病患者外周血,分离单核细胞,制备总RNA,经逆转录合成GPC-3cDNA,以荧光定量PCR扩增并分析其对肝癌诊断与转移监测的价值。结果外周血单核细胞中GPC-3 mRNA阳性仅见肝癌患者,55例肝癌患者中39例阳性(70.9%);肝硬化、肝炎、转移性肝癌、非肝肿瘤患者及正常对照组中未检出阳性(χ2=26.773,P<0.001)。外周血单核细胞GPC-3 mRNA阳性表达与HBsAg阳性(χ2=14.601,P<0.001)、肝癌的TNM分期(χ2=15.252,P<0.001)、门静脉癌栓(χ2=45.658,P<0.001)及伴肝外转移显著相关(χ2=22.273,P<0.001),与瘤体直径、数目、AFP浓度及分化程度间无明显相关;与血AFP可互补诊断,提高肝癌诊断阳性率(90.9%)。结论循环血单核细胞GPC-3 mRNA分析,有助于肝癌诊断和肝外转移监测,对AFP阴性肝癌具互补诊断价值。

     

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  • 出版日期:  2012-10-20
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