PDF下载 ( 1574 KB)
Reliability of metabolic and pathologic parameters in the high fat diet-induced nonalcoholic fatty liver disease rat model established by gastric perfusion
-
摘要:
目的研究高脂乳剂灌胃构建大鼠非酒精性脂肪性肝病(NAFLD)模型的可靠性。方法成年Wistar雄性大鼠47只,体重(170±15)g,随机分为正常组、模型组。所有大鼠给予基础饲料,同时应用高脂乳剂灌胃法喂养模型组大鼠,而对照组大鼠用生理盐水灌胃替代。在4周、8周、12周三个时间段,光镜观察肝组织病理形态学改变;分别检测各组体重和肝脏湿重,计算肝指数(LI);检测大鼠空腹血清ALT和AST水平,观察是否存在组间差异;结果与对照组对比,模型组12周LI显著增大(P=0.000);12周时,血清ALT(P=0.030)及AST(P=0.000)较同期对照组升高;病理结果显示模型组4周肝小叶腺泡3区肝细胞少量脂肪空泡形成;模型组8周肝小叶腺泡3区肝细胞弥漫性脂肪空泡形成,少量炎性细胞浸润;模型组12周肝小叶腺泡3区弥漫性脂肪空泡形成,大量炎性细胞浸润,符合NAFLD典型的病理特征。本实验大鼠死亡率2%,模型构建成功率98%。结论脂肪乳剂灌胃12周可以成功复制大鼠NAFLD模型,该模型具有较强的临床相似性、可靠性。
Abstract:Objective To evaluate the reliability of metabolic and pathologic parameters in the high fat diet-induced nonalcoholic fatty liver disease (NAFLD) rat model using a gastric perfusion method.Methods Forty-seven male Wistar rats were randomly assigned to either the normal control group (N) or the NAFLD model group (M) .There was no significant difference in body weight between the two groups.All the rats were housed under specific pathogen-free (SPF) conditions and acclimated with a normal diet for one week.Afterwards, the M group was administered a high fat diet by gastric perfusion, and the N group was administered 0.95% NS.Rats from both groups were sacrificed at the end of the 4th, 8th, and 12th week for analysis.Body length and weight was recorded for each rat, as well as the liver weight for calculating the liver Index (LI) .Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by standard biochemical methods.Formalin-fixed liver sections were stained with hematoxylin-eosin and were graded for the degree of fatty composition and inflammation.Results Two rats died during the experiment, so that only 45 were included in the analysis.The M group showed pathological changes that were typical of NAFLD, and the degree of fat degeneration was homogeneous.Compared with the N group, the M group had significantly higher LI (P=0.000) , ALT level (P=0.006) , and AST level (P=0.000) .Conclusion A NAFLD model can be successfully induced by 12 weeks of gastric perfusion with high fat diet in adult rats.The metabolic and pathologic parameters produced by this method are consistent with NAFLD.
-
Key words:
- fatty liver /
- animal model /
- high-fat diet
-
[1]聂小燕，张瑜，毛新民，等.非酒精性脂肪肝大鼠模型的建立[J].新疆中医药，2007 , 25 (2) ;7-9. [2]钱晓武，范建高，汪晓红.改良蛋氨酸胆碱缺乏饮食喂养的非酒精性脂肪性肝炎大鼠模型的建立[J].世界华人消化杂志，2007，15 (28) :983一989. [3]孙大裕.脂肪肝动物模型的研究进展[J].中华消化杂志，2002，22 (5) :305一306. [4] 中华医学会肝病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南 (2010修订版) [J].中华肝脏病杂志，2010，18 (3) :163一166. [5]苏慧敏，张知新，潘琳，等.高脂饮食诱导幼鼠脂肪肝模型的建立及其相关代谢研究[J].中华肝脏病杂志，2010, 18 (1) ;54-58. [6]Valenti L, Fracanzani AL, Dongiovanni P，et al. Tummor necroses factor alpha promoter poly-morPhisms and insulin resistance in non-alcoholic fatty liver disease[J].Gastroentr erology, 2002，122 (2) :274一280. [7]Hatsugai K, Ohkohchi N，Fukumori T, et al. Mechanism of Primary graftnon function in a rat model for fatty liver trans plantation[ J].Transpl Int, 2000，13 (suppl1) :s583-589. [8]Ackerman Zvi，Herman Oron，Grozovski M，et al. Fructose-inaucea fatty liver disease hepatic effects of blood pressure and plasma triglyceride reduuction[ J].Hypertension，2005，45 (5) :1012-1018. [9]Terrazos-Luch J，Corona Garcia S, Zentellade PI na M，et al.Butylated hydroxytoluene prevents hepatic damage induced by food oil[J].Proe west pharmaeol Soc, 1997，40 ; 97-99. [10]Zhang BH，Weltman M，Farrel GC. Does steatohepatitis impair liver regeneration? A study in a dietary model of nonalco holic Steatohepatitis in rats[J].Gastroenterol Hepatol，1999，14 (2) :133-137. [11]Leclerca I，Horsmans Y, Desager JP, et al. Dietary restriction of energy and sugar results in a reduction in human cytochrome P450-2E1 activity[J].Br J Nutr, 1999, 82 (4) :257-262. -
本文二维码
计量
- 文章访问数: 295
- HTML全文浏览量: 16
- PDF下载量: 251
- 被引次数: 0
下载:
