Abstract: Objective To clear preliminary clinical characteristics of the infection of HCV genotype 1 and non-genotype 1 in China.Methods Through a national multi-center, large sample epidemiological survey, certified by an international third-party testing organization qualifications, using the internationally accepted method for sequencing analysis of viral genotype, and further on the clinical characteristics of the infection of HCV genotype 1 and non-genotype 1 were analyzed.Results Plasma samples of 764 patients from 18 research centers of China were collected.384 patients were maleand 380 were female, and the mean age was 44.9 ± 14.3 years, the mean duration was 7.7 ± 7.3 years.By analyzing the clinical features, we found that, in the age distribution and duration of disease, the genotype 1 virus infected patients have a greater age and longer duration than that in non-genotype 1 infected patients.The comparison of the ALT levels in patients with a cross-sectional survey showed that 54.9% with abnormal ALT in genotype 1 patients, and 56.9% in non-genotype 1 patients, There was no significant difference (P>0.05) .The comparison of BMI levels in patients showed that abnormal BMI was 39.6% of the patients infected with genotype 1 HCV and 39.6% non-genotype 1.There was no significant difference (P>0.05) .We divided the routes of infection into the blood transfusion infection and Non-transfusion routes of infection.In the patients with blood transfusion infection, 66.1% were genotype 1, while in the patients with non-infected blood transfusion, non-genotype 1 infection accounted for 51.1%.There was a significant difference (P<0.05) .Conclusion Compared with HCV non-genotype 1, HCV genotype 1 infected patients have greater age distribution and longer duration, mostly of them were blood transfusions.