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Screening Chinese medicine components for inhibition of human hepatic stellate cell migration
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摘要: 目的从6种已证实有抗肝纤维化作用的中药组分中,筛选出抑制肝星状细胞迁移的最佳者。方法培养人肝星状细胞株（LX-2）,用细胞迁移杯培养方法分别观察不同浓度梯度（10-4、10-5、10-6、10-7mol/L）的青蒿琥酯、灯盏花素、红景天苷、丹参酚酸B盐、粉防己碱、黄芪总甙对血小板衍生生长因子-BB（PDGF-BB）（10 ng/ml）诱导的LX-2迁移的抑制,从而筛选出各组分最佳作用浓度;随后比较各组分最佳浓度对LX-2迁移的抑制作用,而筛选出最佳组分。结果 6种中药组分均可抑制PDGF诱导的LX-2的迁移;但10-4mol/L丹参酚酸B盐、10-4mol/L黄芪总甙、10-4mol/L与10-5mol/L粉防己碱对LX-2形态有不同程度的影响;依照药物筛选原则,最终选出10-4mol/L青蒿琥酯、10-4mol/L灯盏花素、10-5mol/L红景天甙、10-5mol/L丹参酚酸B盐、10-6mol/L粉防己碱、10-6mol/L黄芪总甙为最佳浓度;将各组分最佳浓度进行统一比较证实,10-5mol/L红景天苷具有更好的抑制迁移作用。结论 10-5mol/L红景天苷抑制PDGF诱导的LX...Abstract: Objective To screen the best component from six of components of traditional Chinese medicine which were confirmed with the anti-fibrosis function.Methods A trans-well chamber system was used to observe the changes of the human hepatic stellated cell line (LX-2) migration.We select human HSC cell line (LX-2) which induced by platelet derived growth factor-BB (PDGF-BB) to study the effect of inhibition of migration.Cells were incubated with 10-4mol/L-10-7mol/L concentration of Artesunate, Breviscapine, Salidroside, Salvianolic acid B, Tetrandrine, Astragalosides, respectively.Results Six kinds of Chinese medicine components can inhibit the PDGF-induced migration of LX-2.10-4mol/L Salvianolic acid B, 10-4mol/L Astragalosides, 10-4mol/L Tetrandrine and10-5mol/L Tetrandrine have different affectiveness on cell morphology.Based on the principles of the selection, 10-4mol/L Artesunate, 10-4mol/L Breviscapine, 10-5mol/L Salidroside, 10-5mol/L Salvianolic acid B, 10-6mol/L Tetrandrine and 10-6mol/L Astragalosides can significantly inhibit LX-2 migration.Of six of them, 10-5mol/L Salvianolic has the best effectiveness (P<0.05) .Conclusion 10-5mol/L Salidroside is the most effective drugs that inhibit the migration of LX-2.
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