慢性乙型肝炎患者外周血趋化因子CXCL10和CXCL11的表达及意义

祝先进; 宋艳芳; 曹颖平; 许伟群; 张臣青

慢性乙型肝炎患者外周血趋化因子CXCL10和CXCL11的表达及意义

1. 福建医科大学附属协和医院检验科
2. 福建中医学院附属人民医院检验科
3. 福建医科大学免疫学系

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中图分类号: R512.62
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出版历程
- 出版日期: 2011-08-20

The expression and significance of CXCL10 and CXCL11 in chronic hepatitis B patients

摘要

摘要: 目的探讨慢性乙型肝炎（CHB）患者外周血中趋化因子CXCL10和CXCL11的表达水平及与ALT和HBV DNA的关系。方法用ELISA方法定量检测CHB患者外周血中CXCL10和CXCL11含量;以实时定量PCR检测CHB患者HBV DNA含量。以全自动生化分析仪检测CHB患者血清中ALT含量。结果 CHB患者血清中CXCL10和CXCL11浓度分别为（123.38±88.37）pg/ml和（129.24±82.79）pg/ml,均高于正常对照组（P<0.05）,且与ALT呈正相关（r值分别为0.739、0.597,P<0.05）;但外周血中CX-CL10和CXCL11的含量与血清中HBV DNA含量无明显相关（r值分别为0.244、0.242,P>0.05）。结论 CHB患者外周血中CX-CL10和CXCL11介导了肝脏炎症细胞浸润和免疫损伤,参与了乙型肝炎慢性化病程。
- 肝炎,乙型,慢性 /
- 趋化因子CXCL10 /
- 趋化因子CXCL11
Abstract: Objective To study the expression levels of CXCL10 and CXCL11 and its relationships with alanine aminotransferase (ALT) and HBV DNA in patients with chronic hepatitis B.Methods The serum levels of CXCL10 and CXCL11 were measured by enzyme-linked immunosorbent assay (ELISA) , the levels of HBV DNA were detected by real-time quantitative polymerase chain reaction (RT-PCR) .Serum ALT were tested with automatic biochemistry analyzer.Results The serum levels of CXCL10 and CXCL11 in the patients with chronic hepatitis B were (123.38±88.37) pg/ml and (129.24±82.79) pg/ml respectively, which was significantly higher than those in normal controls (P<0.05) .In patients with chronic hepatitis B, the serum levels of CXCL10 and CXCL11 were significantly correlated with the serum level of ALT (r=0.739 and 0.597 respectively, P<0.05) , while wasn't correlated with the serum level of HBV DNA (r=0.244 and 0.242 respectively, P>0.05) .Conclusion CXCL10 and CXCL11 play an important role in inflammatory and immune injury, which mediate the chronicity of hepatitis B.
- hepatitis B /
- chronic /
- chemokine CXCL10

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参考文献(10)

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