莪术黄芪超滤膜提取物对人肝癌细胞株HepG2凋亡的诱导作用

岳伟; 付伟; 毛小荣; 张晶

莪术黄芪超滤膜提取物对人肝癌细胞株HepG2凋亡的诱导作用

1. 兰州大学第一医院
2. 甘肃中医学院

详细信息

中图分类号: R735.7
计量
- 文章访问数: 4145
- HTML全文浏览量: 11
- PDF下载量: 912
- 被引次数: 0
出版历程
- 出版日期: 2011-05-20

Study on apoptosis induction of the ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous in HepG2 cell

摘要

摘要: 目的观察莪术黄芪超滤膜提取物对人肝癌细胞株HepG2是否具有凋亡诱导作用。方法四氮唑蓝比色(MTT)法观察莪术黄芪超滤膜提取物对人肝癌细胞株HepG2细胞的抑制率;应用流式细胞术,DNA凝胶电泳方法检测细胞凋亡的发生。结果 (1)莪术黄芪超滤膜提取物对人肝癌细胞株HepG2的生长有明显的抑制作用,与对照组比较差异有统计学意义(P<0.01),抑制作用与剂量及时间呈显著性正相关;(2)莪术黄芪超滤膜提取物诱导人肝癌细胞株HepG2发生凋亡,1.6 g/L处理,72 h效果最佳。结论莪术黄芪超滤膜提取物抑制人肝癌细胞株HepG2生长并促其凋亡。
- 中草药 /
- 肝肿瘤 /
- 细胞凋亡 /
- 肿瘤细胞,培养的 /
- 莪术黄芪超滤膜提取物
Abstract: Objective To observe the effect of the ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous in inducing apoptosis in human HepG2 cell.Methods HepG2 cell proliferation was detected by MTT colorimetry.Apoptosis was observed by flow cytometry and agarose gel electrophoresis.Results The ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous could significantly inhibit HepG2 cell proliferation and there was a significant difference compared with that in the control groups (P<0.01) .There was a remarkable positive correlation between drug concentration and time and the inhibitory rates;The ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous could induce apoptosis in HepG2 cell.The best efficient treatment is the concentration of 1.6g/L for 72h.Conclusion The ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous could induce apoptosis in HepG2 cell.
- Chinese herbal /
- liver neoplasms /
- apoptosis /
- tumor cells /
- cultured

HTML全文

参考文献(5)

[1] 董敏, 胡明月, 郑巧敏, 等.细胞凋亡基因与肿瘤[J].中华医学实践杂志, 2003, 2 (7) :83-86.

[2]汪朝阳, 黄秀榕, 祁明信, 等.中医中药对细胞凋亡的影响研究进展[J].中国中西医结合杂志, 2000, 20 (9) :712-714.

[3]Liu QH, Liu SY, Qi H, et al.Preliminary study on the mechanism of apoptosis in Ehrlich ascites tumor cells by sonochemical activated hematoporphyrin[J].Acta Zool Sinica, 2005, 51 (6) :1073-1079.

[4]Tan C, Dlugosz PJ, Peng J, et al.Auto-activation of the apoptosis protein Bax increases mitochondrial membrane permeability and is inhibited by Bcl-2[J].J Biol Chem, 2006, 281 (21) :14764-14775.

[5]Liu R, Hu YY, Liu C, et al.Multicenter clinical study on Fuzhenghuayu capsule against liver fibrosis due to chronic hepatitis B[J].World J Gastroenterol, 2005, 11 (9) :2892-2899.

施引文献

资源附件(0)

访问统计