高脂饮食致大鼠非酒精性脂肪性肝炎肝纤维化模型的建立

孙林林; 石军; 郝菁华; 任万华; 阎春英; 张捷; 林晓燕; 崔彬

高脂饮食致大鼠非酒精性脂肪性肝炎肝纤维化模型的建立

1. 山东大学附属省立医院肝病中心
2. 山东大学附属省立医院临床科研中心
3. 山东大学附属省立医院病理科

基金项目:

山东省博士基金计划课题(2006BS03027)；

详细信息

中图分类号: R575.2;R-332
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- 出版日期: 2011-03-20

Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

Research funding:

摘要

摘要: 目的建立并研究高脂肪饮食导致大鼠非酒精性脂肪性肝炎(NASH)肝纤维化的动物模型。方法雄性SD大鼠60只,随机分成正常对照组30只和模型组30只,分别给予普通饲料与高脂饲料,于第4、8、12周末将正常对照组与模型组随机处死10只。分别检测血清学指标,取肝组织做HE染色及Masson染色,应用图像分析法对Masson染色进行纤维化面积定量分析。结果两组大鼠体重均增加,8周时肝指数明显升高,血清胆固醇及总甘油三酯8周开始与正常对照组比较差异具有统计学意义。转氨酶不同时间点均有升高,8、12周时与正常对照组比较差异具有统计学意义。高脂饮食4周末,HE染色可见肝小叶结构完整,汇管区见少量炎症细胞浸润,无病理性纤维化发生。8周末,达到脂肪肝的诊断标准,开始出现肝纤维化趋势。造模12周,大鼠肝组织脂肪变程度达重度,纤维化面积增大。结论经改良配方后,成功建立了大鼠NASH肝纤维化模型。
- 肝硬化,实验性 /
- 疾病模型,动物 /
- 高脂饮食
Abstract: Objective This study was aimed to establish the rat model of nonalcoholic steatohepatitis/hepatic fibrosis induced by high-fat diet.Methods Sixty male Sprague-Dawley rats(N=60) were divided into control group(n=30) and model group(n=30) randomly.The rats of control group were fed with common forage and those of the model group were given high-fat diet.Ten rats in each group were terminated at 4,8 and 12 week respectively.The serum-markers were detected.The degrees of hepatic steatosis,inflammation and fibrosis were observed by HE staining and Masson staining.Masson staining was analyzed by image analysis system to achieve the fibrosis area quantitative analysis.Results The weight of Rats in both of these two groups increased.Compared to the control group,the liver index(liver weight/ body weight %),serum cholesterol,triglyceride and aminotransferase levels in the model group increased significantly after 8 weeks after which,the rats in model group were diagnosed with fatty liver disease.After 12 weeks high-fat diet,the pathological injury of the rats hepatic tissue became more and more severe,and resulted into liver fibrosis.Conclusion The rat model of nonalcoholic steatohepatitis/hepatic fibrosis has been established successfully by high-fat diet.
- liver cirrhosis /
- experimental /
- disease models

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参考文献(21)

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高脂饮食致大鼠非酒精性脂肪性肝炎肝纤维化模型的建立

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Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

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