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Effects of knocking down IGFBPrP1 with siRNA on extracellular matrix secretion in HSC-T6
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摘要: 目的利用化学合成的siRNA抑制肝星状细胞（HSC）中胰岛素样生长因子结合蛋白相关蛋白1（IGFBPrP1）基因的表达,观察其对HSC产生细胞外基质（ECM）的影响,以明确IGFBPrP1在肝纤维化中的作用地位。方法 （1）化学合成2对针对IGFBPrP1基因的siRNAs,转染肝星状细胞株HSC-T6,筛选抑制率较高的siRNA用于干扰实验;（2）将HSC-T6分为3组:正常对照组、阴性对照组和IGFBPrP1 siRNA干扰组。将筛选的抑制效率较高的siRNA转染HSC-T6,Western Blot检测其IGFBPrP1、Ⅰ型胶原（ColⅠ）和纤维连接蛋白（FN）的表达。采用单因素方差分析,多重比较采用SNK-q检验。结果 （1）将2对IGFBPrP1 siRNA转染HSC,筛选出能高效抑制IGFBPrP1表达的siRNA;（2）与正常对照组和阴性对照组相比,IGFBPrP1 siRNA干扰组能明显抑制IGFBPrP1、ColⅠ和FN的表达（F=48.018、81.246、43.850,P<0.01）。结论 IGFBPrP1 siRNA能特异性抑制HSC中IGFBPrP1的...
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关键词:
- RNA,小分子干扰 /
- 胰岛素样生长因子结合蛋白质类 /
- 肝细胞 /
- 细胞外基质
Abstract: Objective To investigate the effects of inhibiting insulin-like growth factor binding protein related protein1 (IGFBPrP1) by chemically synthesized small interfering RNA (siRNA) on the secretion of extracellular matrix (ECM) in HSC-T6 and to determine the effect of IGFBPrP1 on the hepatic fibrosis.Methods (1) Two pairs of chemically synthesized siRNAs targeting IGFBPrP1 were respectively transfected into HSC-T6 cells for evaluation of silence efficacy and the one with higher efficacy was used in the following experiments; (2) Divide the HSC-T6 cells into three groups as follows: normal control group, negative control group and IGFBPrP1 siRNA group.HSC-T6 was treated by IGFBPrP1 siRNA, then the expressions of IGFBPrP1 and collagenⅠand fibronectin were detected by Western Blot.The data was analyzed by one-way ANOVA and SNK-q test.Results (1) Two pairs of siRNAs were transfected into HSC-T6.One pair of siRNA, which can highly effectively inhibit expression of IGFBPrP1 gene was screened successfully; (2) The expressions of IGFBPrP1 and collagenⅠand fibronectin in IGFBPrP1 siRNA group were significantly decreased than those in normal control group and negative control group (F﹦48.018, 81.246, 43.850, P﹤0.01) .Conclusion siRNA targeting IGFBPrP1 specifically inhibits the expression of IGFBPrP1 in HSC resulting in decreased synthesis and secretion of collagenⅠand fibronectin in hepatic stellate cells.So IGFBPrP1 can play an important role in liver fibrosis.-
Key words:
- RNA /
- small interfering /
- insulin-like growth factor binding proteins /
- hepatocytes
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[1]Wells RG.Cellular sources of extracellular matrix in hepatic fibrosis[J].Clin Liver Dis, 2008, 12 (4) :759-768. [2]刘毅, 刘立新, 郭晓红, 等.IGFBP-rP1对肝星状细胞活化及核因子-κB活性的影响[J].中国病理生理杂志, 2008, 24 (9) :1789-1793. [3] 张骞骞, 刘立新, 郭晓红, 等.胰岛素样生长因子结合蛋白7在活化肝星状细胞促肝纤维化中的作用[J].中华消化外科杂志, 2009, 8 (6) :457-459. [4]Corey DR.Chemical modification:the key to clinical application of RNA interference?[J].J Clin Invest, 2007, 117 (12) :3615-3622. [5]Kim D, Rossi J.RNAi mechanisms and applications[J].Biotechniques, 2008, 44 (5) :613-616. [6]Tacke F, Weiskirchen R.Liver fibrosis-Pathogenesis and novel therapeutic approaches[J].Internist (Berl) , 2010, 51 (1) :21-29. [7]Friedman SL.Mechanisms of hepatic fibrogenesis[J].Gastroenterology, 2008, 134 (6) :1655-1669. [8]Atzori L, Poli G, Perra A.Hepatic stellate cell:A star cell in the liver[J].Int J Biochem Cell Biol, 2009, 41 (8-9) :1639-1642. -
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