Annexin Ⅱ和Ki-67在胆管癌中的表达及意义

黄波; 赵国忠

Annexin Ⅱ和Ki-67在胆管癌中的表达及意义

黄波,
赵国忠

1. 宁夏医科大学研究生学院
2. 宁夏医科大学附属医院肝胆外科

详细信息

中图分类号: R735.8
计量
- 文章访问数: 264
- HTML全文浏览量: 14
- PDF下载量: 124
- 被引次数: 0
出版历程
- 出版日期: 2011-02-20

Expressions of Annexin Ⅱ and Ki-6 in cholangiocarcinoma and their clinicopathological significance

摘要

摘要: 目的分析胆管癌组织中膜粘连蛋白Ⅱ（AnnexinⅡ）和细胞核增殖抗原（Ki-67）的表达,并探讨其与胆管癌临床病理特征之间的关系。方法运用免疫组织化学染色法检测AnnexinA2和Ki-67蛋白在58例胆管癌组织和癌旁胆管组织及15例非肿瘤胆管组织中的表达,结合临床病理学资料进行统计分析。结果 AnnexinⅡ在三种组织中阳性表达率分别为74.1%（43/58）、31.0%（18/58）、13.3%（2/15）,差异有统计学意义。Ki-67在三种组织中阳性表达率分别为81.0%（47/58）、37.9%（22/58）、6.7%（1/15）,其阳性表达在三种组织之间均有统计学意义。AnnexinⅡ和Ki-67蛋白阳性表达与胆管癌的组织学分级、临床分期和淋巴结转移有关。胆管癌中AnnexinⅡ和Ki-67蛋白表达呈正相关。结论 AnnexinⅡ、Ki-67与胆管癌的发生、发展和淋巴结转移有关,可作为胆管癌诊断的辅助指标,综合应用二项指标对评价胆管癌的恶性程度及预后可能有一定的参考价值。
- 胆管肿瘤 /
- 层黏连蛋白 /
- Ki-67抗原
Abstract: Objective To investigate the expression of AnnexinⅡand Ki-67 in cholangio-carcinoma and their correlations with clinical and pathological features.Methods 58 samples of cholangiocarcinoma and its surrounding tissue and 15 samples of non-carcinoma bile duct tissue were obtained and the expressions of AnnexinⅡand Ki-67 were detected by immunohistochemistry method.Statistical analysis was performed with reference to the related clinical and pathological feature.Results The positive rates of AnnexinⅡ expression in cholangiocarcinoma, tissue near by carcinoma and non-carcinoma bile duct tissue were 74.1% (43/58) , 31.0% (18/58) and 13.3% (2/15) respectively.There was significant difference among the groups.The positive rates of Ki-67 expression in the three tissues were 81.0% (47/58) , 37.9% (22/58) , 6.7% (1/15) , the positive expressions of Ki-67 among the three groups had statistically significant differences.The positive expression of AnnexinⅡ and Ki-67 are correlated with the pathological grades, clinical stages and lymph node metastasis of cholangiocarcinoma.There is positive correlation between the expression rate of AnnexinⅡ and that of Ki-67 in cholangiocarcinoma.Conclusion The positive expression of AnnexinⅡ and that of Ki-67 may be correlated with the carcinogenesis, development and lymph node metastasis of cholangiocarcinoma.They may serve as an auxiliary marker of cholangiocarcinoma.The combine use of the above markers possibly has certain reference significance in evaluating the malignancy of cholangiocarcinoma and its prognosis.
- bile duct neoplasms /
- laminin /
- Ki-67 antigen

HTML全文

参考文献(17)

[1]陈孝平, 陈汉.肝胆外科学[M].北京:人民卫生出版社, 2005:481-482.

[2]Blechacz BR, Gores GJ.Cholangiocarcinoma[J].ClinLiver Dis, 2008, 12 (1) :131-150.

[3]Tanaka T, Akatsuka S, Ozeki M, et al.Redox regulationof annexin 2 and its implications for oxidative stress-induced renal carcinogenesis and metastasis[J].Oncogene, 2004, 23 (22) :3980-3989.

[4]Huang Y, Jin Y, Yan CH, et al.Involvement of AnnexinA2in P53 induced apoptosis in lung cancer[J].Mol CellBiochem, 2008, 309 (1/2) :117-123.

[5]Vilar E, Salazar R, Pérez-García J, et al.Chemothe-rapy and role of the proliferation marker Ki-67 indigestive neuroendocrine tumors[J].Endocr RelatCancer, 2007, 14 (2) :221-232.

[6]纪小龙, 施作霖.诊断免疫组织化学[M].北京:军事医学科学出版社, 1997:12-31.

[7]Burke EC, Jarnagin WR, Hochwald SN, et al.Hilarcholangiocarcinoma:patterns of spread, the importanceof hepatic resection for curative operation, and apresurgical clinical staging system[J].Ann Surg, 1998, 228 (3) :385-394.

[8]Isa T, Tomita S, Nakaehi A, et al.Analysis of microsatelliteinstability, K-ras gene mutation and P53 protein overexpression intrahepatic cholangiocarcinoma[J].Hepatogastroenterology, 2002, 49 (3) :604-608.

[9]Luo W, Yan G, Li L, et al.Epstein-Barr virus latentmembrane protein 1 mediates serine 25 phosphorylationand nuclear entry of Annexin A2 via PI-PLC-PKCα/PKCβpathway[J].Mol Carcinog, 2008, 47 (12) :934-946.

[10]Barwe SP, Anilkumar G, Moon SY, et al.Novel role for Na, K-ATPase in phosphatidylinositol 3-kinase signalingand suppression of cell motility[J].Mol Biol Cell, 2005, 16 (3) :1082-1094.

[11]赵玲, 崔全才.Annexin A2在肿瘤发生中的作用研究进展[J].中华病理学杂志, 2007, 36 (2) :129-132.

[12]萧笑, 王元, 张思河.钙依赖性磷脂结合蛋白AnnexinⅡ的研究进展[J].第四军医大学学报, 2007, 28 (6) :570-573.

[13]Chetcuti A, Margan S H, Russell P, et al.Loss ofannexinⅡheavy and light chains in prostate cancer andits precursors[J].Cancer Res, 2001, 61 (3) :6331-6334.

[14]宋魏, 仇晓菲.AnnexinⅡ与肿瘤的研究进展[J].医学综述, 2008, 14 (6) :851-853.

[15]Scholzen T, Gerdes J.The Ki-67 protein:from the knownand the unknown[J].J Cell Physiol, 2000, 182 (3) :311-322.

[16]Pinder SE, Wencyk P, Sibbering DM, et al.Assessment ofthe new proliferation marker MIB1 in breast carcinomausing image analysis:associations with other prognosticfactors and survival[J].Br J Cancer, 1995, 71 (1) :146-149.

[17]Schindlbeck C, Hantschmann P, Zerzer M, et al.Prognostic impact of KI67, p53, human epithelialgrowth factor receptor 2, topoisomerase IIα, epidermalgrowth factor receptor, and nm23 expression of ovariancarcinomas and disseminated tumor cells in the bonemarrow[J].Cancer, 2007, 17 (5) :1047-1055.

施引文献

资源附件(0)

访问统计