不同基因型丙型肝炎病毒Core蛋白HLA-DRB1*0311、0401表位预测

程玲; 洪国祜; 郭艳; 毛青

不同基因型丙型肝炎病毒Core蛋白HLA-DRB1*0311、0401表位预测

第三军医大学西南医院全军感染病研究所

基金项目:

国家自然科学基金(30771840)；

详细信息

中图分类号: R512.63
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出版历程
- 出版日期: 2011-01-20

Prediction of HLA-DRB1*0311/0401 in recognizing epitopes of HCV Core protein of different genotypes

Research funding:

摘要

摘要:
目的预测各型丙型肝炎病毒（HCV）Core区的人类白细胞抗原（HLA）-DRB1*0311及0401抗原表位。方法从NCBI数据库中检索出十条包含六种分型的HCV完整氨基酸序列,使用生物信息学工具剪切Core区氨基酸序列并对其二级结构、可塑性、亲水性和抗原性指数进行预测,选择可能区域预测HLA-DRB1*0311及0401的抗原表位。结果 HCV Core区二级结构、可塑性、亲水性和抗原性指数预测分析可认为16～28、60～74、98～120区域或为抗原表位区域。HLA-DRB1*0311预测中,HCV-1a/b、4a、6a预测评分高的区域为28～36,HCV-2a/b为63～71,HCV-3a为29～37,HCV-5a为94～102。HLA-DRB1*0401中,HCV-1a、2b、4a、6a预测评分高区域为84～92,HCV～5a为120～128,HCV-1b中120～128可以预测到表位但评分低。结论 HCV Core区HLA-Ⅱ类分子抗原表位具有型间差异。
- 肝炎病毒属 /
- 病毒核心蛋白质类 /
- 基因型 /
- 表位
Abstract:
Objective To predict the epitopes of hepatitis C virus (HCV) core protein of different genotypes, which can be recognized by HLA-DRB1*0311/0401.Methods Ten complete amino acid sequences of HCV were searched from NCBI database, which contain six genotypes of HCV.Core sequences were selected to predict secondary structure, flexiblity, hydrophilicity, antigen index and then predict the HLA-DRB1*0311/0401 in recognizing epitopes.Results The probable regions of epitopes according to the secondary structure, flexiblity, hydrophilcity and antigen index of HCV core protein were 16-28, 60-74 and 98-120 respectively.For HLA-DRB1*0311, the highest scoring region in 1a/1b, 4a, 6a was 28-36, 2a/b was 63-71, 3a was 29-37 and 5a was 94-102.For HLA-DRB1*0401, the highest scoring region in 1a, 2b, 4a, 6a was 84-92, 5a was 120-128, 1b was 120-128 with low score.Conclusion There were different epitopes of HCV Core protein recognized by HLA-Ⅱ between the different HCV genotypes.
- hepacivirus /
- viral core proteins /
- genotype /
- epitopes

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参考文献(13)

[1]McKiernan SM, Hagan R, Curry M, et al.Distinct MHC class I and II alleles are associated with hepatitis C viral clearance, originating from a single source[J].Hepatology, 2004, 40 (1) :108-114.

[2]Ait-Goughoulte M, Banerjee A, Meyer K, et al.Hepatitis C virus core protein interacts with fibrinogen-beta and attenuates cytokine stimulated acute-phase response[J].Hepatology, 2010, 51 (5) :1505-1513.

[3]Drozina G, Kohoutek J, Jabrane-Ferrat N, et al, Expression of MHC II genes[J].Curr Top Microbiol Immunol, 2005, 290:147-170.

[4]Pybus OG, Barnes E, Taggart R, et al.Genetic history of hepatitis C virus in East Asia[J].J Virol, 2009, 83 (2) :1071-1082.

[5]Poynard T, Yuen MF, Ratziu V, et al.Viral hepatitis C[J].Lancet, 2003, 362 (9401) :2095-2100.

[6]Simmonds P.Genetic diversity and evolution of hepatitis C virus--15 years on[J].J Gen Virol, 2004, 85 (11) :3173-3188.

[7]Akuta N, Suzuki F, Sezaki H, et al.Association of amino acid substitution pattern in core protein of hepatitis C virus genotype 2a high viral load and virological response to interferon-ribavirin combination therapy[J].Intervirology, 2009, 52 (6) :301-309.

[8]Alter MJ, Kruszon-Moran D, Nainan OV, et al.The prevalence of hepatitis C virus infection in the United States, 1988 through 1994[J].N Engl J Med, 1999, 341 (8) :556-562.

[9]Esteban JI, Sauleda S, Quer J, et al.The changing epidemiology of hepatitis C virus infection in Europe[J].J Hepatol, 2008, 48 (1) :148-162.

[10]Chen, YD, Liu MY, Yu WL, et al.Hepatitis C virus infections and genotypes in China[J].Hepatobiliary Pancreat[J].Dis Int, 2002, 1 (2) :194-201.

[11]孙亚臣, 岳希全, 孟晓明, 等.RFLP法检测PBMCS和血浆中HCV基因型的相关性与慢性化的关系[J].中国实验诊断学, 2007, 2 (2) :205-207.

[12]Thomas DL, Thio CL, Martin MP, et al.Genetic variation in IL28B and spontaneous clearance of hepatitis C virus[J].Nature, 2009, 461 (7265) :798-801.

[13]Ge D, Fellay J, Thompson AJ, et al.Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance[J].Nature, 2009, 461 (7262) :399-401.

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