肝纤维化大鼠肝组织中NF-κB及ERK-1mRNA的表达

胡泰洪; 蒋祥虎; 蒋跃明; 周敏; 徐敏; 朱锐; 杨玲

肝纤维化大鼠肝组织中NF-κB及ERK-1mRNA的表达

1. 无锡市传染病医院
2. 华中科技大学同济医学院附属协和医院

基金项目:

江苏省中医药管理局资助(No:H05096)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2009-01-20

Expression of NF-κB and ERK-1mRNA in rat with liver fibrosis

Research funding:

摘要

摘要: 目的探讨核转录因子-κB（NF-κB）及细胞外信号调节激酶（ERK）mRNA在CCl4致实验性肝纤维化大鼠肝组织中的表达及其与肝纤维化的关系。方法建立CCl4致大鼠实验性肝纤维化的模型,用免疫组化法比较正常组,模型组的α平滑肌肌动蛋白（α-SMA）的表达,用RT-PCR法比较各组肝组织NF-κB以及ERK-1mRNA的表达。结果模型组中,ERK-1及NF-κBmRNA的表达均增高。结论ERK通路介导的促增殖作用与NF-κB通路介导的促炎症反应,促进HSC的激活、增殖,在肝纤维化的发生发展中均是不可或缺的。
- 核转录因子κB /
- 细胞外信号调节激酶-1 /
- 肝纤维化
Abstract: Objective To evaluate Expression of NF-κB and ERK-1mRNA in rat with liver fibrosis.Methods Liver fibrosis in rats were induced by carbon tetrachloride (CCl4) .Rats were divided into two groups: normal control group, CCl4 model group.The expression of alpha-Smooth Muscle Action were detected by immunohistochemistry.The mRNA expression of nuclear factor kappaB (NF-κB) and extracellular signal-regulated kinase (ERK-1) were determined by RT-PCR.Results The mRNA expression of NF-κB and ERK-1 were significantly higher in model group (P<0.01) .Conclusion The activation of ERK signal transduction pathway enhances HSC proliferation of rats;on the other hand, the enhanced signal of nuclear factor kappaB may accelerate the procedure of liver fibrosis.Both of them may play an important role in liver fibrogenesis of rat.
- NF-κB /
- ERK-1 /
- liver fibrosis

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参考文献(8)

[1]梁增文, 张国, 王天才.大鼠肝纤维化中细胞外信号调节激酶的作用[J].世界华人消化杂志, 2003, 11 (6) ∶730-732.

[2]张晓岚, 宋梅, 白云, 等.细胞外信号调节激酶1与肝星状细胞增殖关系的体内实验研究[J].胃肠病学和肝病学杂志, 2003, 12 (5) ∶440-443.

[3]Feng DY, Zheng H, Tan Y, et al.Effect of phosphorylation ofMAPK and Stat3 and expression of c-fos and c-jun proteins onhepatocarcinogenesis and their clinical significance[J].World J Gas-troenterol.2001, 7 (1) ∶33-6.

[4]Zhang XL, Liu JM, Yang CC, et al.Dynamic expression of extracel-lular signal-regulated kinase in rat liver tissue during hepatic fibro-genesis[J].World J Gastroenterol, 2006, 12 (39) ∶6376-81.

[5]Son G, Iimuro Y, Seki E, et al.Selective inactivation of NF-kap-paB in the liver using NF-kappaB decoy suppresses CCl4-inducedliver injury and fibrosis[J].Am J Physiol Gastrointest Liver Physiol, 2007, 293 (3) ∶G631-9.

[6]Leclercq IA, Farrell GC, Sempoux C, et al.Curcumin inhibit SNF-kappaB activation and reduces the severity of experimental steato-hepatitis in mice[J].J Hepatol, 2004, 41 (6) ∶926-34.

[7]陈科全, 周宇.核因子NF-B与肝纤维化的关系研究现状[J].国际消化病杂志, 2007, 27 (1) ∶9-12.

[8]Nanji AA, Jokelainen K, Tipoe GL, et al.Curcumin prevents alco-hol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes[J].Am J Physiol Gastrointest LiverPhysiol, 2003, 284 (2) ∶G321-7.

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