五羟色胺对大鼠肝性脑病影响的研究

宋闽宁; 陈福; 宋屿娜; 谢泽金

五羟色胺对大鼠肝性脑病影响的研究

1. 解放军第174医院传染科
2. 厦门大学医学院实验动物室
3. 解放军南京军区福州总医院病理科

基金项目:

厦门市科技局资助项目(3502z20034007)；

详细信息

中图分类号: R747.9
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出版历程
- 出版日期: 2008-02-20

The influence of 5-hydroxytryptamine on rat with hepatic encephalopathy.

Research funding:

摘要

摘要: 目的探讨五羟色胺（5-HT）在大鼠急性肝衰竭（ALF）肝性脑病（HE）发病中的影响。方法应用SD大鼠,随机分为正常对照A,观察组B、C、D,每组20只。B、C、D组分别应用5%硫代乙酰胺（TAA）500mg/kg灌胃,制作ALF HE动物模型。B组和C组分别加用塞庚啶10mg/kg、20mg/kg。各组分阶段观察5-HT,肝生化指标、血氨、内毒素检测值、脑核磁共振及脑组织病理学电镜检查。结果B、C、D组5-HT测定值明显高于正常对照组（t≥3.9,P<0.01）。B、C组死亡率低于D组（x2≥0.63,P<0.05）。D组72小时5-HT值明显高于D组48小时组（t=6.1,P<0.01）。B、C组脑组织电镜检查示神经元细胞肿胀,有髓样小体形成,D组脑组织神经元细胞固缩,染色质增生,髓鞘水肿变性明显,部分有分层断裂。结论肝衰竭肝性脑病时5-HT有异常升高。应用5-HT受体拮抗剂可见脑组织病理损伤较轻,死亡率降低。
- 大鼠 /
- 肝性脑病 /
- 5-羟色胺 /
- 5-羟色胺受体拮抗剂 /
- 脑组织病理
Abstract: Objective To explore the influence of 5-hydroxytryptamine on rat with hepatic encephalopathy (HE) in stage of acute liver failures (ALF) .Methods Eighty Sprague-Dawley rats were divided into four groups randomly: Group A acted as control.Model of rat with HE were induced by 5% thioacetamide (TAA) intragastric administration in group B, C and D.Group B and C were given by 5-HT receptor blocking agent (Cyproheptadine) with 10 mg/kg and 20mg/kg respectively.Each group was detected by liver function tests, 5-HT, blood ammonia, serum endotoxin, brain magnetism resonance and histopathology.Results The level of 5-HT in group B, C and D were higher than those in normal control (t≥3.9, P<0.01) .The mortality in group B and C were both lower than that in group D (x2≥0.63, P<0.05) .The level of 5-HT at 72hour in group D was higher than that at 48-hour. (t=6.1, P<0.01) .The electron microscope showed that the neuron had swollen in groups B and C and marrow-like body had formed, whereas in group D, nerve cell were contracted and chromatin were hyperplasia, myeline sheath were edema and denaturation.Conclusion The level of 5-HT was increased obviously in rat with HE.The histological damage of brain has mitigated after administration with 5-HT receptor blocking agent and the mortality had cut down.
- rat /
- hepatic encephalopathy /
- 5-hydroxytryptamine /
- serotonin receptor /
- histopathology

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参考文献(14)

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