PDF下载 ( 187 KB)
The applied value of Dane granule antigen of hepatitis B viral in detecting chronic hepatitis B infection
-
摘要: 目的分析乙肝病毒五项检测方法和乙肝病毒丹氏颗粒抗原检测试剂在检测慢性乙型肝炎感染中的一致性,探讨丹氏颗粒抗原检测试剂在检测慢性乙肝感染的经济性和可靠性。方法ELISA方法检测134例慢性乙肝患者的血清标志物和丹氏颗粒抗原,荧光定量PCR方法检测HBV DNA。比较各组丹氏颗粒抗原水平及HBVDNA定量水平和乙肝五项的相互关系。结果慢性乙肝患者HBsAg、HBeAg、HBcAb阳性组、HBsAg、HBcAb阳性组、HBsAg、HBeAb、HBcAb阳性组和HBsAb、HBeAb、HBcAb阳性组丹氏颗粒抗原阳性率分别为100.0(、100.0(、94.3(和0。结论丹氏颗粒抗原试剂在检测慢性乙肝感染中与乙肝五项指标及HBV DNA定量之间具有一致性。丹氏颗粒抗原是一个能较好的反应乙肝病毒复制的指标,对乙肝的诊断、预后及抗病毒疗效观察有较好的实用价值。
-
关键词:
- 乙型肝炎病毒 /
- 乙型肝炎病毒丹氏颗粒抗原 /
- 血清标志物 /
- 乙肝病毒核酸
Abstract: Objective To analyze the consistency in detecting method of hepatitis B virus (HBV) and Diagnostic Kit for hepatitis B virus Core Antigen and Presl Antigen (Dane granule antigen) .To explore the economy and credibility of Dane granule antigen of HBV in detecting chronic hepatitis B infection.Methods HBV markers、Dane granule antigen of HBV and HBVDNA in 134 patients with chronic hepatitis B were detected respectively by ELISA and fluorescence quantitative PCR.To compare the relationship among Dane granule antigen、HBVDNA and HBV markers in different groups.Result The positive rates of Dane granule antigen of HBV were 100.0%、100.0%、94.3% and 0% respectively in the groups of HBsAg (+) /HBeAg (+) /HBcAb (+) 、HBsAg (+) /HBcAb (+) 、HBsAg (+) /HBeAb (+) /HBcAb (+) and HBsAb (+) /HBeAb (+) /HBcAb (+) .Conclusion Dane granule antigen of HBV has consistency in detecting the infection of chronic hepatitis B with serum markers and HBV-DNA quantitation.It can reflect the replication of HBV and have applied value in the diagnosis of hepatitis B、judgement of prognosis and the curative effect of antivirus.-
Key words:
- hepatitis B virus /
- Dane granule antigen of hepatitis B virus /
- serum markers /
- HBV-DNA
-
[1]吴晓蔓, 刘海涛.HBV-DNA定量和定性PCR反应及临床实用性的探讨[J].临床肝胆病杂志, 2000, 16 (4) ∶226~227. [2]Neurath AR, Kent SB, Strick N, et al.Identification and chemicalsynthesis of a host cell receptor binding site on hepatitis B virus[J].Cell, 1986, 46∶429-431. [3]张永宏, 赵艳, 胡中杰.乙型肝炎病毒抗原临床意义分析[J].临床荟萃, 2001, 16 (24) ∶1111-1113. [4]冯福民, 米志宝, 王洪莉, 等.前S1蛋白在乙型肝炎诊断、治疗和预后中的作用[J].中西医结合肝病杂志, 1997, 7 (1) ∶18-21. [5]Hu KQ.Occult hepatitis B virus infection and its clinical implica-tions[J].J Viral Hepat, 2002, 9∶243-257. [6]Simon K, Rotter K, Zalewska M, et al.HBV-DNA level in blood-serum as a predictor of geed response to therapy with interferon-al2pha-2b of patients with chronic hepatitis B[J].Med sci Monit, 2000, 6 (5) ∶971~975. [7]苏宝凤, 任健康, 张华, 等.前S1抗原的检测与HBV-DNA及其血清学标志物的相关性探讨[J].现代检验医学杂志, 2005, 20 (2) ∶41-44. [8]唐跃华, 谢健敏, 梁玉全, 等.慢性乙型肝炎患者血清HBeAg、HBV-DNA与肝组织炎症关系的探讨[J].中华传染病杂志, 2005, 23 (5) ∶338-341. [9]王利君, 何思春, 谢元宏, 等.乙型肝炎前S1抗原、HBeAg与HBV-DNA的关系及临床意义[J].四川医学, 2005, 26 (4) ∶453-454. -
本文二维码
计量
- 文章访问数: 2388
- HTML全文浏览量: 11
- PDF下载量: 781
- 被引次数: 0
下载:
