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Affection of the mutations and genotypes of hepatitis B virus precore and basic core promoter on HBeAg expression
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摘要: 研究乙型肝炎病毒前C区和BCP区突变及基因型对HBeAg表达的影响。分别用基因芯片和基因测序的方法检验HBV DNA阳性的乙型肝炎病毒前C区和BCP区突变及基因型,应用时间分辨免疫荧光法检测HBeAg含量,按照有无HBV前C区1896、BCP区1762、1764双突变、基因型等指标进行分组分析。无前C区1896和BCP区1762/1764双突变组、单纯1762、1764双突变和1896突变组以及1896、1762、1764联合突变组之间相互比较,HBeAg含量相差显著,F=6.47,P<0.01;B、C基因型间HBeAg含量相比,相差无显著性,t=0.1394,P>0.05。乙型肝炎病毒前C区和BCP区突变能显著影响HBeAg量的表达,从而导致乙型肝炎病毒致病能力的变化。Abstract: To study the affection of the mutations and genotypes of hepatitis B virus precore and basic core promoter (BCP) on HBeAg expression.Gene chip technique was applied to detect gene mutations in HBV pre-core nt.1896, BCP nt.1762 and 1764.And DNA sequencing technique was used to sequence their genotypes Time-resolution immunofluorescenece technique was used to quantitate HBeAg.Those samples were divided according to the conditions mutations and genotypes of precore and BCP. The quantity of HBeAg were significant difference between single 1762/1764 mutation group, single 1896 mutation group, 1896/1762/1764 unite mutations group and no above mutations group (F=6.47, P<0.01) .There was no significant different between genotype B group and genotype C group in quantity of HBeAg (t=0.1394, P>0.05) .Hepatitis B virus precore and BCP mutations might significantly affect the expression of HBeAg, and lead to the variety of HBV pathogenicity.
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Key words:
- hepatitis B virus /
- basic core promoter (BCP) /
- precore gene /
- mutation
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