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Autoimmune related clinical tests in diagnosis of primary biliary cirrhosis
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摘要: 为研究原发性胆汁性肝硬化（PBC）免疫学指标测定的临床意义,选取32例确诊PBC的住院患者,采用ELISA法检测患者血清中的抗线粒体抗体M2亚型（AMA-M2）;间接免疫荧光法检测抗线粒体抗体（AMA）,抗核抗体（ANA）,抗平滑肌抗体（SMA）;斑点法检测抗肝肾微粒体抗体（LKM-1）,抗肝溶质Ⅰ型抗原抗体（LC-1）,抗可溶性肝抗原/肝胰抗原抗体（SLA/LP）;免疫比浊法检测免疫球蛋白（IgG,IgA,IgM）和类风湿因子（RF）。同时观察AMA-M2与生化肝功指标的关系,比较PBC患者治疗前后AMA-M2的变化。结果显示诊断PBC意义最大的是AMA-M2,其阳性率最高达94%,其次是AMA和ANA,阳性率分别为88%和86%;AMA-M2值的高低与血清转氨酶、碱性磷酸酶和γ-谷氨酰转肽酶无关,熊去氧胆酸治疗前后AMA-M2变化不大。
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关键词:
- 原发性胆汁性肝硬化 /
- 抗线粒体抗体—M2亚型 /
- 熊去氧胆酸
Abstract: Thirty-two patients diagnosed of primary biliary cirrhosis (PBC) were detected antimitochondrial antibody M2 subtype (AMA-M2) by ELISA, antimitochondrial antibody (AMA) and anti-nuclear antibody (ANA) by indirect immune-fluorescence assay, other autoimmune antibody were also detected.It was shown that serum AMA-M2 antibody had the highest sensitivity and specificity of 94% for PBC, higher than AMA and ANA.The level of AMA-M2 had no close relation with the severity of the PBC itself.Furthermore, AMA-M2 did not significantly change after ursodeoxylic acid treatment. -
[1]张福奎, 贾继东, 王宝恩, 等.45例原发性胆汁性肝硬化的临床特征[J].中华内科杂志, 2002, 41 (3) ∶163-167. [2] Heathcote EJ.Managem ent of primary b iliary cirrhosis.The Am eri-can Association for the study of L iver D iseases practice gu idelines[J].Hepatology, 2000, 31 (4) ∶1005-1013. [3] 唐淑珍, 李茁, 尤红, 等.抗线粒体抗体M2亚型在诊断原发性胆汁肝硬化中的意义[J].中华临床医药杂志, 2002, 3 (1) ∶15-17. [4]贾继东.自身抗体检查在自身免疫性肝病诊断中的应用[J].中华检验医学杂志, 2003, 26 (2) ∶120-122. -
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