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Hepatic chymase content in chronic hepatitis and hepatic fibrosis
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摘要: 为探讨肝组织中糜酶（Chymase）浓度在慢性病毒性肝炎肝纤维化中的意义。采用酶联免疫吸附实验（ELISA）检测45例慢性肝炎肝组织中Chymase浓度,观察不同的纤维化分期（S1-S4）,其肝组织中Chymase浓度;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。慢性肝炎纤维化分期重的S3和S4患者,其肝组织中Chymase浓度〔S3+S4=（31.3±24.6）ng/mg〕明显高于纤维化轻的S1和S2患者〔S1+S2=（5.7±4.8）ng/mg〕,P<0.01;免疫组化染色结果,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁,其分布与纤维化部位相一致,且肝组织中Chymase浓度高的患者,其肝组织内Chymase标记的肥大细胞分布增多。推测肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。Abstract: To investigate spatial relatioship betwwen human chymase and fibrosis in liver specimens affected by chronic hepatitis.The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay (ELISA) in 45 cases of chronic hepatitis.Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2 , 〔S3+S4= (31.3±24.6) ng/mg vs.S1+S2= (5.7±4.8) ng/mg, P<0.01〕.Cells immunoreactive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with flbrosis.Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.
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Key words:
- chymase /
- chronic hepatitis /
- mast cell /
- hepatic fibrosis /
- angiotensin
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[1]YoshikawaH, TasakaK.Suppressionofmastcellactivationbyglucocorti coid[J].ArchImmunolTherExp (Wars2) , 2000, 48∶487-495. [2]SatomuraK, ShimizuS, NagatoT, etal.Establishmentofanassaymethod forhumanmastcellchymase[J].ⅡepatolRes, 2002, 24∶361-367. [3]YoshikawaH, TasakaK.Suppressionofmastcellactivationbyglucocorti coid[J].ArchImmunolTherExp (Wars2) , 2000, 48∶487-495. [4]GinsburgH, Ben-DavidE.Mastcellgrowthonfibroblastmonolayers∶two-cellentities[J].Immunology, 1998, 45∶371-380. [5]FarrellDJ, HinesJE, WallsAF, etal.Intrahepaticmastcellsinchronic liverdisease[J].Hepatology, 1995, 22∶1175-1181. [6]TaniK, OgushiF, WawanoT, etal.Chymaseisapotentchemoattractant forhumanmonocytesanneutrophils[J].JleukocBiol, 2000, 67∶585-589. [7]宋怀宇, 韩丽辉、朱菊人.慢性乙型肝炎、肝硬化患者肝组织中肾素-血管紧张素系统mRNA的表达及意义[J].中华肝脏病杂志, 2004, 12 (2) ∶109. [8]YoshijiH, KuriyamaS, YoshiJ, etal.AngiotensinⅡtype1receptorinter actionisamajorforliverfibrosisdevelopmentinrats[J].Hepatology, 2001, 34∶745-747. -
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