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Detection and significance of interleukin 10 levels and HBVDNA in serums from patients with chronic hepatis B .
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摘要: 检测慢性乙型肝炎 （CHB）患者血清IL - 10与HBVDNA、ALT水平 ,探讨乙型肝炎慢性化的机理。检测 77例CHB临床血清标本 ,用实时荧光定量聚合酶链反应 （FQ -PCR）测HBVDNA含量 ;用双抗体夹心ELISA法定量检测血清IL - 10水平。CHB患者血清IL - 10浓度明显高于正常对照组 （P <0 0 1） ,HBVDNA阳性组高于HBVDNA阴性组 ;CHB组患者血清ALT异常者的IL - 10浓度明显高于ALT正常组 ;IL - 10水平与HBVDNA复制程度呈正相关。CHB患者血清IL - 10水平升高与HBV持续感染、HBVDNA高复制合并肝损害有关。Abstract: To understand the impacts of interleukin 10 (IL-10) in serums from patients with chronic hepatitis B (CHB) on the persistent infection of hepatitis B virus (HBV) . The levls of HBVDNA and IL-10 in serums from 77 patients with CHB and 10 normal controls were measured by real time quantitive PCR and ELISA method. The levels of IL-10 in serums was greater in patients with CHB than in controls (P<0.01) . Patients with seropositive for HBV DNA had higher levels of IL-10 in serums than patients with seronegative for HBVDNA. But the levels of IL-10 were higher in chronic hepatitis B patients with ALT abnormal than in patients with ALT normal. IL-10 may have an important role in persistent infection of HBV in patient with CHB.
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Key words:
- hepatitis B /
- interleukin-10 /
- HBV-DNA
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