原因不明性肝炎血清中TTVDNA的检出及其临床意义

潘发愤; 陶志华; 陈晓东; 王忠永; 周祖木; 吴建波; 丁建光; 陈永平; 王震龙

原因不明性肝炎血清中TTVDNA的检出及其临床意义

基金项目:

温州市科委医学科学基金资助 (S2 0 0 0A17)；

详细信息

中图分类号: R512.6
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出版历程
- 出版日期: 2002-04-20

A study on TTV DNA detection and clinical significance in patients with hepatitis of unknown etiology

Research funding:

摘要

摘要: 研究原因不明性肝炎中TTV （Transfusiontransmittedvirus）感染的检出状况及其临床意义。在TTVORF1设计引物 ,建立巢式聚合酶链反应（Nested -PCR） ,检测 5 0例原因不明性肝炎、4 0例正常健康人与 30例乙肝病毒携带者血清中TTVDNA ,比较TTVDNA阳性肝炎患者临床特征。 5 0例原因不明性肝炎中 16例TTVDNA阳性（32 % ） ,比正常健康人群（2 / 4 0 ,5 % ）与乙肝病毒携带者（2 / 30 ,6 7% ）血清中TTVDNA检出率为高（P <0 0 5 ） ,16例TTVDNA阳性患者中以慢性肝炎占多数（12 / 16 ,75 5 % ） ,表现为反复、持续ALT异常。TTV在原因不明性肝炎中感染率明显高于其他人群 ,可能是本地区原因不明性肝炎的致病因子
- 输血传播病毒 /
- 巢式聚合酶链反应DNA /
- 原因不明性肝炎
Abstract: To investigate TTV infection among patients with hepatitis of unknown etiology and its clinical significance. A nested polymerase chain reaction (PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in 50 patients with hepatitis of unknown etiology, 40 health persons and 30 HBV carriers. The positive rate of TTV DNA in patients with hepatitis of unknown etiology, health persons and in HBV carriers were 32% (16/50) , 5% (2/40) and 6 7% (2/30) , respectively. It had statistical difference between them ( P <0 05) . Among 16 patients with TTV DNA positive response, 12 cases were chronic hepatitis (12/16, 75%) . TTV infection would be one of the major causes of hepatitis with unknown etiology in this areas.
- TT virus /
- Nested-PCR /
- Hepatitis with unknown etiology

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参考文献(6)

[1] 病毒性肝炎防治方案 (试行) 中华传染病杂志, 1995, 3∶241-247.

[2]OkamotoH , NishizawaT , KatoN , etal.MolecularcloningandcharacterizationofanovelDNAvirus (TTV) associatedwithpost transfusionhepatitisofunknownetiology[J].HepatologyResearch, 1998, 10∶1-15.

[3]周伯平, 马为明, 孙彤, 等TTV在非甲-庚型肝炎患者中的检测、克隆及测序[J].中华传染病杂志, 1998, 16∶131-133.

[4]罗朝霞, 郎振为, 阎慧平, 等地高辛素标记探针原位杂交法检测肝及肝外组织中的TTVDNA[J].中华医学杂志, 2000, 80∶438-439.

[5]黄呈辉, 陈汝光, 何忠平, 等PCR结合斑点杂交法检测血清TTVDNA[J].中华肝脏病杂志, 1999, 7∶110-111.

[6]TanakaH , OkamotoH , etal.InfectionwithanunenvelopedDNAvirus (TTV) associatedwithposttransfusionnon-AtoGhepatitisinhepatitispatientsandhealthyblooddonorsinThailand[J].JMedVirol, 1998, 56∶234-236.

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