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Cloning and sequence analysis of the rat augmenter of liver regeneration genomic DNA
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摘要: 根据大鼠肝再生增强因子 (Augmenterofliverregeneration ,ALR)cDNA序列设计特异性引物 ,采用多聚酶链反应 (PCR)法自Wistar大鼠基因组DNA中扩增出大鼠ALR基因组DNA ,全长为 15 0 8nbp ,结构为 3个外显子和 2个内含子 ,外显子长度分别为 18bp、197bp和 16 3bp ,各编码 6个、6 4个和 5 5个氨基酸残基 ,长度与小鼠、人类ALR的外显子一致。Abstract: WT5”BZ]To investigate the structure of the genomic DNA sequence for ALR, specific PCR method was used to sub clone target fragment from the Wistar rat genome The whole length is 1508bp, including 3 exons and 2 introns The exons are 18bp, 197bp and 163bp, each one encode 6aa, 64aa and 55aa respectively The lengths of the exon are as long as that of human and mouse [WT5”HZ]
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Key words:
- WT5”BZ]Augmenter of liver regeneration /
- Polymerase chain reaction /
- Exon /
- Intron
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[1]HagiyaM , FrancavillaA , PolimegeL , etal Cloningandsequenceanalysisoftheaugmenterofliverregeneration (ALR) gene:expres sionofbiologicallyactiverecombinantALRanddemonstrationoftis suedistribution[J] PNASUSA , 1994, 91∶814 2 [][2 ]成军 , 陈菊梅 肝再生增强因子研究进展 [J] 肝脏 , 1999, 4∶117 [][3 ]成军 , 钟彦伟 , 刘妍 , 等 人肝再生增强因子基因组DNA的克隆化与序列分析 [J] 中华肝脏病杂志 , 2 0 0 0 , 8∶12 -
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