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实验性肝纤维化时MMP-1、TIMP-1的表达与Ⅰ、Ⅲ型胶原含量变化的关系
Relativity of Expression of MMP-1、TIMP-1 and Variability of Type Ⅰ、Ⅲ Collagen During Experimental Liver Fibrosis
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摘要: 为了观察实验性肝纤维化时MMP - 1、TIMP - 1的表达与Ⅰ、Ⅲ型胶原含量变化之间的关系 ,本文制备了免疫性大鼠肝纤维化模型 ,运用定量RT -PCR检测肝脏内MMP - 1、TIMP - 1的表达 ,通过免疫组化检测肝组织中Ⅰ、Ⅲ型胶原的含量。运用相关理论作统计学分析。结果发现在肝纤维化发生时 ,Ⅰ型胶原和Ⅲ型胶原含量的变化有显著相关性 ;MMP - 1的表达在肝纤维化组与正常对照组之间未见显著差异 ,与Ⅰ、Ⅲ型胶原的含量变化之间也未见有相关性 ;TIMP - 1的表达在肝纤维化发生时显著增高 ,与Ⅰ、Ⅲ型胶原的变化也有显著相关性。结果表明肝纤维化发生时MMP - 1的表达未见明显改变 ,而TIMP - 1的表达显著增高 ,且与Ⅰ、Ⅲ型胶原含量的增高呈显著相关 ,提示TIMP - 1的表达增高在肝纤维化的发生、发展中具有重要作用
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关键词:
- 肝纤维化 /
- 基质金属蛋白酶-1 /
- 反义金属蛋白酶组织抑制因子-1 /
- Ⅰ型胶原 /
- Ⅲ型胶原
Abstract: To observe the relativity of expression of MMP-1、TIMP-1 and variability of type Ⅰ and Ⅲ collagen during experimental liver fibrosis, we made the rats liver fibrosis model by immune attack of human serum albumin (HSB) , and detected the expression of MMP-1 and TIMP-1 by quantitative RT-PCR, assayed the type I and type Ⅲ collagen by immunohistochemistry, then analyzed relativity of expression of MMP-1、TIMP-1 and variability of typeⅠ、Ⅲ collagen during liver fibrosis We found that the change of content of type Ⅰ and type Ⅲ collagen presented a high relativity (r=0 875, P<0 001) ;no difference was found between normal group and fibrosis group in the expression of MMP-1, and no relativity was found between the expression of MMP-1 and the change of content of type Ⅰ、type Ⅲ collagen;but we found the expression of TIMP-1 became significantly high after formation of liver fibrosis (P<0 01) , and it has the high relativity to the variability of type Ⅰand Ⅲ collagen So we got the conclusion that the expression of MMP-1 has no significant change during liver fibrosis, but that of TIMP-1 become obvious high, and there is a distinct relativity between the expression of TIMP-1 and the increasing of type Ⅰ、type Ⅲ collagen during liver fibrosis All of these suggest that increasing of the expression of TIMP-1 play a important role in the formation of liver fibrosis -
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