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血清非对称性二甲基精氨酸在非酒精性脂肪性肝病大鼠模型肝脏炎症活动进程中的变化

丁洁 王亮 李娟 孔银 赵睿 何晶晶 李光明 王娟霞 张岭漪

引用本文:
Citation:

血清非对称性二甲基精氨酸在非酒精性脂肪性肝病大鼠模型肝脏炎症活动进程中的变化

DOI: 10.3969/j.issn.1001-5256.2016.01.029
基金项目: 

2014年甘肃省自然科学基金项目(145RJZA147); 中央高校基本科研业务费专项资金(laujbky-2013-215); 

详细信息
  • 中图分类号: R575.5;R-332

Changes in serum asymmetric dimethylarginine levels in progression of liver inflammation in an experimental rat model of nonalcoholic fatty liver disease

Research funding: 

 

  • 摘要:

    目的利用非酒精性脂肪性肝病(NAFLD)大鼠模型观察血清非对称性二甲基精氨酸(ADMA)在NAFLD炎症进程的变化。方法肝组织标本及血清标本分别来自实验组高脂乳剂灌胃法成功构建的NAFLD模型(M组)及同期生理盐水灌胃替代构建的正常对照组(C组)。分别检测M组4、8、12周(M4、M8、M12)和C组12周(C12)大鼠血清ALT、ADMA水平及肝组织匀浆一氧化氮(NO)水平,同期评估各组肝脂肪炎症进程积分(NAS)。数据服从正态分布且方差齐,多组间比较应用单因素方差分析,进一步两两比较应用LSD-t法;服从正态分布且方差不齐,多组间比较应用Kruskal-Wallis H检验;相关性分析应用Pearson相关法。结果与C12组相比,M12组大鼠血清ALT、ADMA水平及肝组织匀浆NO水平显著升高,差异有统计学意义(P值分别为0.010、<0.001、<0.001)。血清ADMA与ALT水平无明显相关性(r=0.195,P=0.228),与肝组织匀浆NO水平、大鼠NAS呈正相关(r=0.631,P<0.001;r=0.782,P<0.001)。结论 NAFLD大...

     

  • [1]KOCAK H,GUMUSLU S,ERMIS C,et al.Oxidative stress and asymmetric dimethylarginine is independently associated with carotid intima media thickness in peritoneal dialysis patients[J].Am J Nephrol,2008,28(1):91-96.
    [2]WANG XY,ZHAO J,YANG HW.Asymmetrical dimethylarginine antagonizes glutamate-induced apoptosis in PC12 cells[J].J Mol Neurosci,2013,49(1):89-95.
    [3]NDER A,AYCAN Z,KOCA C,et al.Evaluation of asymmetric dimethylarginine(ADMA)levels in children with growth hormone deficiency[J].J Clin Res Pediatr Endocrinol,2014,6(1):22-27.
    [4]DING J,WANG L,LI J,et al.Reliability of metabolic and pathologic parameters in the high fat diet-induced nonalcoholic fatty liver disease rat model established by gastric perfusion[J].J Clin Hepatol,2012,28(7):531-534.(in Chinese)丁洁,王亮,李娟,等.高脂乳剂灌胃构建大鼠非酒精性脂肪性肝病模型可靠性的再研究[J].临床肝胆病杂志,2012,28(7):531-534.
    [5]Group of Fatty Liver and Alcoholic Liver Diseases,Society of Hepatology,Chinese Medical Association.Guidelines for management of non-alcoholic fatty liver disease[J].J Clin Hepatol,2010,26(2):120-124.(in Chinese)中华医学会肝脏病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南[J].临床肝胆病杂志,2010,26(2):120-124.[·6]TANRIKULU-KK S,KOAK H,NER-IYIDOGˇAN Y,et al.Serum fetuin-A and arginase-1 in human obesity model:is there any interaction between inflammatory status and arginine metabolism?[J].Scand J Clin Lab Invest,2015,75(4):301-307.
    [7]FERRIGNO A,DI PASQUA LG,BERARDO C,et al.Liver plays a central role in asymmetric dimethylarginine-mediated organ injury[J].World J Gastroenterol,2015,21(17):5131-5137.
    [8]MOOKERJEE RP,MEHTA G,BALASUBRAMANIYAN V,et al.Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension[J].J Hepatol,2015,62(2):325-331.
    [9]SERVILLO L,GIOVANE A,CAUTELA D,et al.The methylarginines NMMA,ADMA,and SDMA are ubiquitous constituents of the main vegetables of human nutrition[J].Nitric Oxide,2013,30:43-48.
    [10]KATUNGA LA,GUDIMELLA P,EFIRD JT,et al.Obesity in a model of gpx4 haploinsufficiency uncovers a causal role for lipidderived aldehydes in human metabolic disease and cardiomyopathy[J].Mol Metab,2015,4(6):493-506.
    [11]DAVIDS M,RICHIR MC,VISSER M,et al.Role of dimethylarginine dimethylaminohydrolase activity in regulation of tissue and plasma concentrations of asymmetric dimethylarginine in an animal model of prolonged critical illness[J].Metabolism,2012,61(4):482-490.
    [12]DOGRU T,GENC H,TAPAN S,et al.Plasma fetuin-A is associated with endothelial dysfunction and subclinical atherosclerosis in subjects with nonalcoholic fatty liver disease[J].Clin Endocrinol(Oxf),2013,78(5):712-717.
    [13]DOGRU T,GENC H,TAPAN S,et al.Elevated asymmetric dimethylarginine in plasma:an early marker for endothelial dysfunction in non-alcoholic fatty liver disease?[J].Diabetes Res Clin Pract,2012,96(1):47-52.
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  • 收稿日期:  2015-06-18
  • 出版日期:  2016-01-20
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