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维生素D相关基因单核苷酸多态性与HBV相关慢加急性肝衰竭的相关性分析
Association of vitamin D-related gene polymorphisms with hepatitis B virus-related acute-on-chronic liver failure
文章发布日期:2019年05月13日  来源:  作者:欧伟杰,林烨鸿,周卿,等  点击次数:346次  下载次数:84次

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【摘要】:目的探究维生素D相关基因单核苷酸多态性(SNP)在HBV相关慢加急性肝衰竭(ACLF)患者中的表达情况及其与HBV-ACLF短期预后的关系。方法纳入福建医科大学附属第一医院肝病中心于2012年7月-2018年3月收治的慢性乙型肝炎患者400例,根据其病程中是否发生ACLF,分为HBV-ACLF组(n=122)和Non-ACLF组(n=278)。分别收集两组患者的临床资料及实验室检查结果,同时采集外周血标本,应用imLDRTM多重SNP分型试剂盒检测维生素D受体(VDR)基因rs1544410、rs2228570及维生素D结合蛋白(VDBP)基因rs2282679共3个位点的SNP表达分型;并对HBV-ACLF组患者进行随访观察,追踪其3个月的生存情况。对比分析维生素D相关基因SNP与HBV-ACLF易感性及预后的关系。采用Hardy-Weinberg平衡检验评估各组基因样本的群体代表性。对于满足正态分布的计量资料,2组间比较采用t检验;对于不满足正态分布的计量资料,2组间比较采用Mann-Whitney U检验。SNP与HBV-ACLF发生的相对风险度采用多元logistic 回归分析。结果HBV-ACLF组患者与Non-ACLF组患者在VDR基因的rs1544410、rs2228570、VDBP基因rs2282679位点基因型及等位基因分布频率之间的差异均无统计学意义(P值均> 0.05);另外VDR基因rs1544410、rs2228570及VDBP基因rs2282679基因型及其等位基因的分布频率在HBV-ACLF存活组和死亡组患者之间的差异均无统计学意义(P值均>0.05)。结论VDR基因rs1544410、rs2228570及VDBP基因rs2282679共3个位点的SNP与HBV-ACLF的发生及短期预后之间不存在相关关系。
【Abstract】:ObjectiveTo investigate the expression of single nucleotide polymorphisms (SNP) of vitamin D-related genes in patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) and its association with short-term prognosis of HBV-ACLF. MethodsThe patients with chronic hepatitis B who were admitted to Liver Diseases Research Center, The First Affiliated Hospital of Fujian Medical University, from July 2012 to March 2018 were enrolled, and according to the presence or absence of acute-on-chronic liver failure during the course of the disease(n=400), they were divided into HBV-ACLF group(n=122) and non-ACLF group(n=278). The clinical data and laboratory examination results were collected; peripheral blood samples were collected, and imLDRTM multiple SNP typing kit was used to determine the genotypes of the SNPs of vitamin D receptor (VDR) rs1544410 and rs2228570 and vitamin D-binding protein (VDBP) rs2282679. The patients in the HBV-ACLF group were followed up for 3 months to observe their survival. The association of vitamin D-related gene SNP with HBV-ACLF susceptibility and prognosis was analyzed. Hardy-Weinberg equilibrium was used to evaluate the representativeness of gene samples. The t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. A logistic regression analysis was used to investigate the odds ratio of SNP in the development of HBV-ACLF. ResultsThere were no significant differences in the genotype and allele frequencies of VDR rs1544410 and rs2228570 and VDBP rs2282679 between the HBV-ACLF group and the non-ACLF group (all P>0.05). In addition, there were also no significant differences in the genotype and allele frequencies of VDR rs1544410 and rs2228570 and VDBP rs2282679 between the HBV-ACLF patients who survived and those who died (all P>0.05). ConclusionSNPs of VDR rs1544410 and rs2228570 and VDBP rs2282679 are not associated with the development and short-term prognosis of HBV-ACLF.
【关键字】:受体, 骨化三醇; 维生素D结合蛋白质; 乙型肝炎病毒; 慢加急性肝功能衰竭; 多态性, 单核苷酸
【Key words】:receptor, calcitriol; vitamin D-binding protein; hepatitis B virus; acute-on-chronic liver failure; polymorphism, single nucleotide
【引证本文】:OU WJ, LIN YH, ZHOU Q, et al. Association of vitamin D-related gene polymorphisms with hepatitis B virus-related acute-on-chronic liver failure[J]. J Clin Hepatol, 2019, 35(6): 1308-1312. (in Chinese)
欧伟杰, 林烨鸿, 周卿, 等. 维生素D相关基因单核苷酸多态性与HBV相关慢加急性肝衰竭的相关性分析[J]. 临床肝胆病杂志, 2019, 35(6): 1308-1312.

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