首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 2019年 6期 HBV cccDNA研究进展 => 专家论坛 =>HBV cccDNA..
HBV cccDNA定量检测方法的研究进展
Recent advances of quantitative detection methods for HBV cccDNA
文章发布日期:2019年05月13日  来源:  作者:江培学,毛日成,张继明  点击次数:309次  下载次数:105次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:从慢性HBV感染的肝细胞中清除HBV cccDNA被认为是根除HBV的关键。在抗病毒治疗之前、期间和之后监测HBV cccDNA对于慢性乙型肝炎患者的疗效评估极为重要;随着靶向HBV cccDNA的抗HBV新药研发的不断推进,也迫切需要准确而灵敏的HBV cccDNA检测方法,以评价其疗效。近年来,为了提高HBV cccDNA定量检测的特异度,在传统的PCR定量检测方法的基础上进行了改进。同时,为了提高敏感度,还推出了HBV cccDNA的数字PCR定量检测方法等。本文综述了引入酶切的qPCR法、磁珠捕获杂交法、滚环扩增结合原位PCR法、数字PCR法和单细胞内数字PCR法等方法在 HBV cccDNA定量检测方面的应用进展。
【Abstract】:Elimination of HBV cccDNA from hepatocytes infected with chronic HBV virus is considered to be the key to eradicating HBV. Monitoring HBV cccDNA before, during, and after viral treatment is essential for routine treatment of patients with chronic hepatitis B. With the introduction of new anti-HBV treatment technologies and new drugs targeting HBV cccDNA, Accurate and sensitive HBV cccDNA assays are urgently needed to evaluate efficacy. In recent years, HBV cccDNA detection methods have achieved gratifying results in both traditional PCR methods and digital PCR methods popular in recent years. In this paper, the advances in HBV cccDNA quantitative detection by qPCR, Magnetic bead capture hybridization, rolling circle amplification combined with in situ PCR, digital PCR and digital PCR assay in single cells were reviewed.
【关键字】:乙型肝炎病毒; 共价闭合环状DNA; 检测方法
【Key words】:hepatitis B virus; cccDNA; quantitative detection method
【引证本文】:JIANG PX, MAO RC, ZHANG JM. Recent advances of quantitative detection methods for HBV cccDNA[J]. J Clin Hepatol, 2019, 35(6): 1201-1204. (in Chinese)
江培学, 毛日成, 张继明. HBV cccDNA定量检测方法的研究进展[J]. 临床肝胆病杂志, 2019, 35(6): 1201-1204.

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号